Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS26420 and PFLU_RS26425 are separated by 136 nucleotidesPFLU_RS26425 and PFLU_RS26430 are separated by 48 nucleotidesPFLU_RS26430 and PFLU_RS26435 are separated by 160 nucleotidesPFLU_RS26435 and PFLU_RS26440 are separated by 89 nucleotides PFLU_RS26420: PFLU_RS26420 - TIGR03862 family flavoprotein, at 5,908,685 to 5,909,911 _RS26420 PFLU_RS26425: PFLU_RS26425 - DEAD/DEAH box helicase, at 5,910,048 to 5,911,382 _RS26425 PFLU_RS26430: PFLU_RS26430 - drug/metabolite exporter YedA, at 5,911,431 to 5,912,327 _RS26430 PFLU_RS26435: PFLU_RS26435 - Lrp/AsnC family transcriptional regulator, at 5,912,488 to 5,912,949 _RS26435 PFLU_RS26440: PFLU_RS26440 - acyl carrier protein, at 5,913,039 to 5,913,302 _RS26440
Group Condition PFLU_RS26420 PFLU_RS26425 PFLU_RS26430 PFLU_RS26435 PFLU_RS26440
stress Betaine (C)(N); with MOPS; with chloride +0.1 -1.4 -0.0 -0.2 -2.2
nitrogen source NAG (N); with MOPS -0.3 -0.7 -0.2 +0.2 -1.8
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.2 +0.2 -0.0 -0.1 -2.4
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS -0.1 -1.3 +0.1 -0.1 -0.9
no stress control Glucose (C) and nitrate (N); with MOPS -0.1 -0.0 -0.1 -0.4 -1.6
carbon source L-Valine (C) -0.3 -0.8 -0.2 +0.2 -1.1
carbon source L-Carnitine (C) -0.6 -0.7 -0.1 -0.1 -0.7
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS +0.0 -1.2 +0.0 -0.3 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.1 -0.6 -0.1 -0.4 -0.9
stress Betaine (C)(N); with MOPS; with chloride +0.1 +0.1 +0.1 -0.3 -1.7
no stress control Glucose (C) and L-Glutamine (N); with TAPS +0.2 -1.0 +0.2 -0.3 -0.6
stress malate (C) and Ammonium chloride (N); with TAPS -0.0 +0.0 -0.1 +0.4 -1.8
carbon source Phloretic Acid 2 mM (C) +0.1 -0.0 -0.0 +0.6 -1.9
carbon source Inosine 10 mM (C) +0.3 -0.0 +0.1 +0.1 -1.7
stress L-Glutamine (C)(N); with TAPS; with chloride +0.6 +0.2 -0.0 -0.3 -1.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.8 -1.0 -0.0 +0.2 +0.4
stress Betaine (C)(N); with MOPS; with chloride +0.1 -1.0 +0.2 +0.3 -0.7
carbon source acetate 30 mM (C) +0.3 +0.4 -0.1 -0.4 -1.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 +0.4 -0.1 +0.3 -1.7
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.3 +0.7 -0.3 -0.2 -0.7
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.5 -0.8 -0.0 +0.5 +0.5
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.4 +1.4 +0.1 +0.0 -0.7
carbon source Quinic Acid (C) -0.1 -0.5 -0.2 +0.3 +1.1
pH Growth at pH9 and (C) Trisodium citrate +0.8 -0.4 -0.3 -0.2 +0.6
carbon source p-Coumaric 2 mM (C) -0.2 +0.4 -0.2 -0.4 +1.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.1 -0.7 +0.1 +0.5 +0.7
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 -0.5 +0.0 -0.3 +1.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.4 +0.2 +0.3 +0.2 +0.9
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.5 +1.3 +0.1 +0.4 +0.4
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.3 +1.5 -0.2 -0.2 +1.2
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