Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS24675 and PFLU_RS24680 are separated by 331 nucleotidesPFLU_RS24680 and PFLU_RS24685 are separated by 4 nucleotidesPFLU_RS24685 and PFLU_RS24690 are separated by 287 nucleotidesPFLU_RS24690 and PFLU_RS24695 are separated by 63 nucleotides PFLU_RS24675: PFLU_RS24675 - protein killer suppression protein, at 5,525,224 to 5,525,502 _RS24675 PFLU_RS24680: PFLU_RS24680 - NIF3 1, at 5,525,834 to 5,526,145 _RS24680 PFLU_RS24685: PFLU_RS24685 - phosphoribosylformylglycinamidine synthase, at 5,526,150 to 5,530,046 _RS24685 PFLU_RS24690: PFLU_RS24690 - membrane-bound lytic murein transglycosylase MltF, at 5,530,334 to 5,531,794 _RS24690 PFLU_RS24695: PFLU_RS24695 - PTS glucose transporter subunit IIB, at 5,531,858 to 5,532,136 _RS24695
Group Condition PFLU_RS24675 PFLU_RS24680 PFLU_RS24685 PFLU_RS24690 PFLU_RS24695
no stress control Glycine betaine (C)(N); with TAPS +0.6 -0.4 N.D. -1.1 -5.4
no stress control L-Glutamine (C)(N); with MOPS -0.5 -0.3 N.D. -0.2 -5.1
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.0 -0.9 N.D. +0.1 -5.2
carbon source D-Trehalose 5 mM (C) +0.1 -0.6 N.D. +0.3 -5.1
carbon source D-Trehalose 5 mM (C) +0.1 -0.3 N.D. +0.3 -5.1
carbon source D-Trehalose 5 mM (C) +0.1 -0.0 N.D. +0.3 -5.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.0 -1.1 N.D. -1.5 -1.9
carbon source Choline chloride 10 mM (C) +0.2 +0.1 N.D. -1.0 -3.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.4 -0.7 N.D. -2.2 -0.6
stress Sucrose (C) and Ammonium chloride (N); with TAPS -0.1 +0.3 N.D. -0.2 -3.8
carbon source Phloretic Acid 2 mM (C) -0.2 +0.9 N.D. +0.7 -5.1
carbon source D-Trehalose (C) +0.2 -0.3 N.D. +0.4 -3.8
carbon source D,L-Malic Acid (C) +0.2 +1.2 N.D. -0.0 -4.9
carbon and nitrogen source NAG carbon and (N) -0.2 -0.8 N.D. +2.3 -4.7
carbon source D-Glucose (C); with MOPS +0.1 +0.6 N.D. +0.4 -3.3
nitrogen source NAG (N) -0.1 -0.6 N.D. +1.2 -2.1
carbon source p-Coumaric (C) 5 mM -0.1 -0.6 N.D. -1.6 +1.9
carbon source L-Arabinose (C); with MOPS +0.6 -0.3 N.D. +1.3 -1.8
carbon source 4-Aminobutyric (C) -0.4 -1.9 N.D. +1.9 +0.3
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.7 +1.0 N.D. +1.2 -1.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.3 -2.2 N.D. +0.8 +1.3
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.4 +1.1 N.D. -1.8 +1.4
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -0.3 -1.5 N.D. +1.2 +1.1
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.1 +0.5 N.D. -1.7 +1.9
carbon source Inosine (C) +0.2 +1.2 N.D. +1.4 -1.9
pH Growth at pH9 and (C) D-Glucose +0.5 -0.4 N.D. +2.4 -1.2
carbon and nitrogen source NAG carbon and (N) +0.1 -0.9 N.D. +2.4 +1.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 +0.6 N.D. +1.0 +2.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.9 +1.2 N.D. +1.1 +0.7
carbon source acetate 30 mM (C) +0.2 +0.5 N.D. +2.0 +1.7
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