Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS24495 and PFLU_RS24500 are separated by 315 nucleotidesPFLU_RS24500 and PFLU_RS24505 are separated by 279 nucleotidesPFLU_RS24505 and PFLU_RS24510 overlap by 1 nucleotidesPFLU_RS24510 and PFLU_RS24515 are separated by 136 nucleotides PFLU_RS24495: PFLU_RS24495 - tRNA-Met, at 5,488,630 to 5,488,706 _RS24495 PFLU_RS24500: PFLU_RS24500 - tRNA-Met, at 5,489,022 to 5,489,098 _RS24500 PFLU_RS24505: PFLU_RS24505 - bifunctional lysylphosphatidylglycerol flippase/synthetase MprF, at 5,489,378 to 5,492,020 _RS24505 PFLU_RS24510: PFLU_RS24510 - virulence factor family protein, at 5,492,020 to 5,493,306 _RS24510 PFLU_RS24515: PFLU_RS24515 - single-stranded-DNA-specific exonuclease RecJ, at 5,493,443 to 5,495,152 _RS24515
Group Condition PFLU_RS24495 PFLU_RS24500 PFLU_RS24505 PFLU_RS24510 PFLU_RS24515
temperature Growth at 34C N.D. N.D. -2.9 -4.1 -0.2
temperature Growth at 34C N.D. N.D. -2.4 -3.9 -0.2
temperature Growth at 34C N.D. N.D. -2.5 -3.3 -0.3
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -2.5 -2.8 +0.2
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -2.2 -2.7 +0.1
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -2.0 -2.6 +0.0
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -2.1 -2.4 +0.1
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -1.1 -1.7 -0.0
temperature Growth at 10C N.D. N.D. -1.3 -1.3 -0.2
temperature Growth at 10C N.D. N.D. -1.2 -1.4 -0.1
temperature Growth at 10C N.D. N.D. -1.1 -1.4 -0.2
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -1.1 -1.5 -0.0
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -1.1 -1.3 +0.1
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -0.4 -1.5 +0.0
carbon source Shikimic Acid (C) N.D. N.D. -0.2 -0.9 -0.5
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -0.1 -1.2 +0.2
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -0.1 -1.0 +0.2
pH Growth at pH6 and (C) D-Glucose N.D. N.D. -0.2 -0.3 -0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride N.D. N.D. +0.0 -0.4 -0.5
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. -0.4 -0.5 +0.1
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS N.D. N.D. -0.1 -0.7 +0.0
carbon source 4-Acetoxy-3-methoxycinnamic 2 mM (C) N.D. N.D. +0.2 -0.6 -0.3
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS N.D. N.D. -0.2 -0.6 +0.1
no stress control L-Glutamine (C)(N); with MOPS N.D. N.D. -0.1 -0.6 +0.4
no stress control Sucrose (C) and Glycine betaine (N); with MOPS N.D. N.D. +0.3 -0.4 +0.2
nitrogen source no extra (N) N.D. N.D. +0.2 +0.6 -0.2
carbon source 2-Deoxy-D-Ribose 10 mM (C) N.D. N.D. -0.0 +0.7 +0.2
carbon source Xanthosine (C) N.D. N.D. +0.1 +0.3 +0.5
nitrogen source no extra (N) N.D. N.D. +0.7 +1.0 -0.0
nitrogen source no extra (N) N.D. N.D. +0.8 +1.2 -0.1
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