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Fitness for 5 genes in
Pseudomonas fluorescens SBW25-INTG
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500 nt
PFLU_RS24065 and PFLU_RS24070 overlap by 1 nucleotides
PFLU_RS24070 and PFLU_RS24075 are separated by 14 nucleotides
PFLU_RS24075 and PFLU_RS24080 are separated by 1 nucleotides
PFLU_RS24080 and PFLU_RS24085 are separated by 151 nucleotides
PFLU_RS24065: PFLU_RS24065 - cell envelope integrity protein TolA, at 5,389,646 to 5,390,719
_RS24065
PFLU_RS24070: PFLU_RS24070 - protein TolR, at 5,390,719 to 5,391,180
_RS24070
PFLU_RS24075: PFLU_RS24075 - protein TolQ, at 5,391,195 to 5,391,890
_RS24075
PFLU_RS24080: PFLU_RS24080 - tol-pal system-associated acyl-CoA thioesterase, at 5,391,892 to 5,392,347
_RS24080
PFLU_RS24085: PFLU_RS24085 - Holliday junction branch migration DNA helicase RuvB, at 5,392,499 to 5,393,557
_RS24085
Group
Condition
PFLU
_RS24065
PFLU
_RS24070
PFLU
_RS24075
PFLU
_RS24080
PFLU
_RS24085
nitrogen source
nitrite (N)
N.D.
N.D.
N.D.
-1.1
-2.5
temperature
Growth at 34C
N.D.
N.D.
N.D.
-1.0
-2.5
temperature
Growth at 34C
N.D.
N.D.
N.D.
-0.8
-2.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
N.D.
N.D.
-0.7
-2.8
nitrogen source
NAG (N)
N.D.
N.D.
N.D.
-0.9
-2.5
temperature
Growth at 34C; with MOPS
N.D.
N.D.
N.D.
-0.7
-2.6
pH
Growth at pH8 and (C) D-Glucose
N.D.
N.D.
N.D.
-0.8
-2.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
N.D.
N.D.
-0.4
-2.9
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
N.D.
N.D.
N.D.
-0.4
-2.9
carbon source
L-Arabinose (C)
N.D.
N.D.
N.D.
-0.9
-2.3
carbon source
NAG (C); with MOPS
N.D.
N.D.
N.D.
-0.8
-2.4
temperature
Growth at 10C
N.D.
N.D.
N.D.
-0.6
-2.5
temperature
Growth at 34C; with MOPS
N.D.
N.D.
N.D.
-0.7
-2.4
no stress control
Sucrose (C) and Glycine betaine (N); with TAPS
N.D.
N.D.
N.D.
-0.1
-3.0
no stress control
Sucrose (C) and Glycine betaine (N); with TAPS
N.D.
N.D.
N.D.
-0.2
-2.9
temperature
Growth at 34C
N.D.
N.D.
N.D.
-0.9
-2.2
carbon source
L-Arabinose (C)
N.D.
N.D.
N.D.
-0.5
-2.5
carbon source
Trisodium citrate (C)
N.D.
N.D.
N.D.
-0.9
-2.1
carbon and nitrogen source
NAG carbon and (N); with MOPS
N.D.
N.D.
N.D.
-1.2
-1.9
nitrogen source
nitrate (N)
N.D.
N.D.
N.D.
-0.6
-2.4
carbon source
L-Arabinose (C)
N.D.
N.D.
N.D.
-0.6
-2.4
carbon and nitrogen source
NAG carbon and (N); with MOPS
N.D.
N.D.
N.D.
-0.5
-2.5
carbon source
NAG (C); with MOPS
N.D.
N.D.
N.D.
-0.3
-2.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
N.D.
N.D.
N.D.
-0.0
-2.9
nitrogen source
Urea (N); with MOPS
N.D.
N.D.
N.D.
-0.8
-2.1
nitrogen source
nitrite (N)
N.D.
N.D.
N.D.
-0.8
-2.1
carbon source
L-Arabinose (C); with MOPS
N.D.
N.D.
N.D.
-1.0
-1.9
pH
Growth at pH8 and (C) D-Glucose
N.D.
N.D.
N.D.
-0.4
-2.5
pH
Growth at pH6 and (C) Trisodium citrate
N.D.
N.D.
N.D.
-0.7
-2.2
carbon source
4-Acetoxy-3-methoxycinnamic (C)
N.D.
N.D.
N.D.
-0.5
-2.5
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