Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS23520 and PFLU_RS23525 are separated by 106 nucleotidesPFLU_RS23525 and PFLU_RS23530 overlap by 102 nucleotidesPFLU_RS23530 and PFLU_RS23535 are separated by 89 nucleotidesPFLU_RS23535 and PFLU_RS23540 are separated by 36 nucleotides PFLU_RS23520: PFLU_RS23520 - LysR family transcriptional regulator, at 5,281,446 to 5,282,342 _RS23520 PFLU_RS23525: PFLU_RS23525 - SDR family oxidoreductase, at 5,282,449 to 5,283,207 _RS23525 PFLU_RS23530: PFLU_RS23530 - multidrug/biocide efflux PACE transporter, at 5,283,106 to 5,283,618 _RS23530 PFLU_RS23535: PFLU_RS23535 - LysR family transcriptional regulator, at 5,283,708 to 5,284,562 _RS23535 PFLU_RS23540: PFLU_RS23540 - RNA polymerase sigma factor, at 5,284,599 to 5,285,822 _RS23540
Group Condition PFLU_RS23520 PFLU_RS23525 PFLU_RS23530 PFLU_RS23535 PFLU_RS23540
carbon source Phloretic Acid 2 mM (C) -0.2 -1.2 -0.1 -0.6 -0.4
carbon source Inosine (C) -0.1 -0.3 +0.0 -0.8 -1.1
carbon source Ferulic Acid 2 mM (C) -0.1 -0.8 -0.2 -0.5 -0.2
stress Betaine (C)(N); with MOPS; with chloride -0.2 -1.3 -0.1 -0.2 -0.0
stress Betaine (C)(N); with MOPS; with chloride -0.0 -0.8 -0.1 -0.5 -0.3
carbon source caffeic 2 mM (C) +0.3 -0.1 -0.3 -0.9 -0.5
carbon source p-Coumaric 2 mM (C) -0.1 -0.9 +0.1 -0.7 +0.2
stress Sucrose (C) and L-Glutamine (N); with MOPS; with PEG -0.4 -0.7 -0.1 +0.1 -0.3
carbon source L-Valine 10 mM (C) -0.1 -1.3 +0.1 -0.2 +0.2
carbon source p-Coumaric 2 mM (C) -0.0 -0.8 +0.1 -0.4 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 +0.4 +0.1 -1.4 -0.5
carbon source L-Valine 10 mM (C) -0.4 -0.8 -0.1 -0.6 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.2 -0.8 +0.1 -0.5 +0.2
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS +0.2 -0.6 +0.1 -0.3 -0.3
carbon source Xanthosine (C) -0.6 -0.3 +0.2 -0.3 +0.2
carbon source NAG (C) -0.5 -0.4 -0.1 +0.4 -0.2
pH Growth at pH9 and (C) Trisodium citrate +0.0 -0.6 -0.2 +0.4 -0.3
no stress control Glycine betaine (C)(N); with TAPS -0.5 -0.6 -0.1 +0.4 +0.2
carbon source 4-Acetoxy-3-methoxycinnamic 2 mM (C) +0.1 -0.8 +0.1 -0.3 +0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.2 +0.1 +0.0 +0.1 -1.0
carbon source Shikimic Acid (C) +0.2 -0.4 +0.4 -0.5 +0.0
carbon source Phloretic Acid 2 mM (C) +0.0 -0.5 -0.1 +0.6 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 -0.4 +0.2 +0.3 -0.5
carbon source L-Isoleucine (C) -0.1 -0.1 -0.1 -0.5 +0.8
pH Growth at pH8 and (C) Trisodium citrate -0.1 -0.7 +0.0 +0.7 +0.3
carbon and nitrogen source NAG carbon and (N) -0.2 -0.2 -0.2 +0.2 +0.6
carbon source 4-Aminobutyric (C) +0.4 +0.2 +0.0 -0.3 +0.5
carbon source L-Isoleucine (C) +0.0 +1.0 +0.1 +0.1 +0.2
carbon source 4-Aminobutyric (C) +0.2 +0.5 -0.1 +0.3 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 +0.6 +0.1 +0.3 +0.3
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