Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS22680 and PFLU_RS22685 are separated by 189 nucleotidesPFLU_RS22685 and PFLU_RS22690 are separated by 42 nucleotidesPFLU_RS22690 and PFLU_RS22695 overlap by 4 nucleotidesPFLU_RS22695 and PFLU_RS22700 are separated by 272 nucleotides PFLU_RS22680: PFLU_RS22680 - Fe/S-dependent 2-methylisocitrate dehydratase AcnD, at 5,106,721 to 5,109,315 _RS22680 PFLU_RS22685: PFLU_RS22685 - 2-methylcitrate synthase, at 5,109,505 to 5,110,632 _RS22685 PFLU_RS22690: PFLU_RS22690 - methylisocitrate lyase, at 5,110,675 to 5,111,568 _RS22690 PFLU_RS22695: PFLU_RS22695 - GntR family transcriptional regulator, at 5,111,565 to 5,112,278 _RS22695 PFLU_RS22700: PFLU_RS22700 - ATP-dependent zinc protease, at 5,112,551 to 5,113,084 _RS22700
Group Condition PFLU_RS22680 PFLU_RS22685 PFLU_RS22690 PFLU_RS22695 PFLU_RS22700
carbon source L-Valine 10 mM (C) -3.3 -3.9 -3.2 -2.9 +0.6
carbon source L-Isoleucine (C) 10 mM -3.7 -4.1 -3.3 -1.7 +0.2
carbon source L-Isoleucine (C) 10 mM -3.5 -3.6 -3.2 -1.7 +0.1
carbon source L-Valine 10 mM (C) -2.6 -3.7 -3.2 -2.5 +0.4
carbon source L-Isoleucine (C) 10 mM -3.4 -4.0 -2.6 -1.4 +0.1
carbon source L-Valine 10 mM (C) -2.7 -3.6 -2.8 -2.5 +0.4
carbon source Citraconic 20 mM (C) -3.0 -3.8 -3.1 -1.4 +0.5
carbon source L-Isoleucine (C) -2.8 -3.1 -2.6 -2.0 +0.1
carbon source L-Valine (C) -2.7 -3.5 -2.2 -1.8 -0.1
carbon source propionate (C) -3.2 -3.6 -3.5 -1.6 +1.8
carbon source Citraconic 20 mM (C) -2.9 -3.2 -3.4 -1.0 +0.3
carbon source Citraconic 20 mM (C) -2.9 -3.7 -3.0 -0.8 +0.4
carbon source propionate 20 mM (C) -3.4 -4.1 -3.6 -0.9 +3.1
carbon source propionate 20 mM (C) -3.2 -4.0 -3.4 -0.4 +2.9
carbon source L-Isoleucine (C) -2.4 -2.1 -2.4 -1.4 +0.4
carbon source L-Valine (C) -1.9 -2.7 -1.8 -1.1 -0.2
carbon source propionate (C) -2.4 -2.4 -2.5 -1.4 +1.1
carbon source propionate (C) -2.6 -2.4 -2.9 -0.6 +1.6
carbon source propionate 20 mM (C) -3.1 -3.1 -2.9 -0.3 +2.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.7 -2.6 -1.4 -0.8 -0.3
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -0.8 -2.0 -2.1 -0.8 +0.1
carbon source L-Isoleucine (C) -1.6 -2.0 -1.5 -1.1 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.1 -2.1 -1.7 -0.2 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.8 -1.8 -1.8 -0.4 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.0 -1.9 -1.1 -0.4 -0.3
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -1.1 -0.1 -2.9 -0.5 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.1 -0.9 -1.2 -0.5 -0.6
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -1.1 -1.6 -1.5 -0.1 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.4 -1.8 -1.2 -0.2 -0.3
no stress control Ferulic (C) and Ammonium chloride (N); with MOPS -0.4 -1.7 -1.0 -0.4 +0.3
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