Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS22410 and PFLU_RS22415 are separated by 222 nucleotidesPFLU_RS22415 and PFLU_RS22420 are separated by 65 nucleotidesPFLU_RS22420 and PFLU_RS22425 are separated by 133 nucleotidesPFLU_RS22425 and PFLU_RS22430 are separated by 42 nucleotides PFLU_RS22410: PFLU_RS22410 - acyl-CoA dehydrogenase, at 5,038,479 to 5,039,627 _RS22410 PFLU_RS22415: PFLU_RS22415 - recombination protein RecR, at 5,039,850 to 5,040,452 _RS22415 PFLU_RS22420: PFLU_RS22420 - NADP-dependent oxidoreductase, at 5,040,518 to 5,041,552 _RS22420 PFLU_RS22425: PFLU_RS22425 - YbaB/EbfC family nucleoid-associated protein, at 5,041,686 to 5,042,024 _RS22425 PFLU_RS22430: PFLU_RS22430 - DNA polymerase III subunit gamma/tau, at 5,042,067 to 5,044,133 _RS22430
Group Condition PFLU_RS22410 PFLU_RS22415 PFLU_RS22420 PFLU_RS22425 PFLU_RS22430
nitrogen source NAG (N); with MOPS -0.5 -0.8 -0.3 N.D. N.D.
pH Growth at pH9 and (C) Trisodium citrate -0.2 -1.2 -0.1 N.D. N.D.
stress L-Glutamine (C)(N); with TAPS; with chloride -0.4 -0.9 -0.2 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.1 -1.2 -0.1 N.D. N.D.
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS -0.3 -1.0 -0.1 N.D. N.D.
pH Growth at pH9 and (C) Trisodium citrate -0.5 -1.0 +0.2 N.D. N.D.
pH Growth at pH8 and (C) Trisodium citrate -0.1 -1.1 +0.0 N.D. N.D.
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.6 -0.7 +0.2 N.D. N.D.
carbon source Shikimic Acid (C) -0.3 -0.8 +0.0 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 -0.9 +0.3 N.D. N.D.
temperature Growth at 25C; with MOPS -0.4 -0.7 +0.2 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.1 -1.1 +0.3 N.D. N.D.
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.4 -0.8 -0.4 N.D. N.D.
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.1 -1.0 +0.2 N.D. N.D.
carbon and nitrogen source NAG carbon and (N) +0.1 -1.0 +0.3 N.D. N.D.
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.9 -0.2 +0.4 N.D. N.D.
carbon source L-Carnitine (C) +0.4 -0.7 -0.1 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 -0.8 +0.4 N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.3 -0.9 +0.1 N.D. N.D.
no stress control Glycine betaine (C)(N); with TAPS +0.4 -0.6 -0.2 N.D. N.D.
carbon source L-Ornithine 10 mM (C) +0.3 -0.7 +0.2 N.D. N.D.
nitrogen source nitrate (N); with MOPS +0.5 -0.9 +0.3 N.D. N.D.
stress Betaine (C)(N); with MOPS; with chloride +0.7 -1.0 +0.2 N.D. N.D.
no stress control Glycine betaine (C)(N); with MOPS +0.6 -0.7 +0.2 N.D. N.D.
stress Betaine (C)(N); with MOPS; with chloride +0.8 -0.5 -0.1 N.D. N.D.
stress Betaine (C)(N); with TAPS; with chloride +0.2 +0.9 -0.1 N.D. N.D.
carbon source Inosine (C) +0.2 +0.8 +0.2 N.D. N.D.
carbon source Quinic Acid (C) +0.3 +0.9 +0.3 N.D. N.D.
carbon source L-Lactate (C) +0.6 +1.0 -0.1 N.D. N.D.
carbon source pyruvate (C) +0.5 +1.0 +0.1 N.D. N.D.
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