Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS22240 and PFLU_RS22245 are separated by 284 nucleotidesPFLU_RS22245 and PFLU_RS22250 are separated by 106 nucleotidesPFLU_RS22250 and PFLU_RS22255 overlap by 7 nucleotidesPFLU_RS22255 and PFLU_RS31775 overlap by 2 nucleotides PFLU_RS22240: PFLU_RS22240 - N-acetyltransferase, at 5,000,259 to 5,001,383 _RS22240 PFLU_RS22245: PFLU_RS22245 - hypothetical protein, at 5,001,668 to 5,003,011 _RS22245 PFLU_RS22250: PFLU_RS22250 - hypothetical protein, at 5,003,118 to 5,003,363 _RS22250 PFLU_RS22255: PFLU_RS22255 - RNA polymerase sigma factor, at 5,003,357 to 5,003,971 _RS22255 PFLU_RS31775: PFLU_RS31775 - hypothetical protein, at 5,003,970 to 5,004,152 _RS31775
Group Condition PFLU_RS22240 PFLU_RS22245 PFLU_RS22250 PFLU_RS22255 PFLU_RS31775
carbon source NAG (C) +0.0 -0.0 -4.0 +0.2 -0.2
carbon source Cytidine 10 mM (C) +0.1 -0.0 -2.5 -0.2 -1.3
nitrogen source no extra (N); with MOPS -0.1 -0.0 -2.6 -0.1 -1.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.0 +0.0 -2.0 +0.1 -1.8
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.3 +0.0 -2.5 +0.0 -1.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.0 -0.0 -2.4 +0.1 -1.2
pH Growth at pH8 and (C) D-Glucose -0.1 -0.2 -1.9 +0.1 -1.3
nitrogen source nitrate (N); with MOPS -0.3 +0.3 -2.4 +0.2 -1.0
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.3 +0.0 -4.0 -0.3 +0.8
carbon source Shikimic Acid (C) -0.1 -0.1 -2.7 +0.1 -0.0
pH Growth at pH6 and (C) D-Glucose -0.2 -0.2 -1.4 -0.1 -0.9
carbon source D-Xylose (C) +0.0 +0.0 -1.3 +0.1 -1.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.2 -0.1 -1.3 -0.0 -1.0
pH Growth at pH9 and (C) D-Glucose +0.2 -0.4 -1.8 +0.0 -0.6
stress Glucose (C) and Betaine (N); with TAPS; with chloride +0.1 +0.0 -0.3 -0.3 -2.1
carbon source L-Valine 10 mM (C) +0.3 +0.0 -1.8 -0.1 -0.9
stress Sucrose (C) and L-Glutamine (N); with MOPS; with PEG +0.1 +0.1 -2.2 -0.1 -0.2
carbon source D-Glucosamine Hydrochloride 10 mM (C) -0.2 -0.1 -2.3 +0.3 +0.0
carbon source L-Carnitine (C) -0.2 -0.3 -2.1 +0.0 +0.3
carbon source Phloretic Acid 2 mM (C) -0.4 +0.4 -1.6 -0.0 -0.5
stress Betaine (C)(N); with MOPS; with chloride -0.2 -0.2 -2.2 +0.1 +0.4
carbon source Phloretic Acid 2 mM (C) +0.6 -0.4 -2.2 +0.4 -0.1
carbon source L-Valine 10 mM (C) +0.3 +0.1 -2.1 +0.3 +0.1
carbon source p-Coumaric 2 mM (C) +0.3 +0.0 -1.5 +0.5 -0.5
pH Growth at pH9 and (C) Trisodium citrate +0.1 -0.1 -2.2 +0.8 +0.5
carbon source p-Coumaric 2 mM (C) +0.3 +0.0 -2.2 +0.2 +1.1
carbon source Xanthosine (C) -0.2 +0.2 +1.5 +0.0 -1.8
carbon source L-Arabinose (C) +0.1 +0.1 +1.0 +0.2 -1.5
stress Betaine (C)(N); with TAPS; with chloride +0.5 -0.0 +0.8 +0.3 -1.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.1 -0.1 +1.7 +0.2 -0.9
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