Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS21110 and PFLU_RS21115 are separated by 121 nucleotidesPFLU_RS21115 and PFLU_RS21120 are separated by 60 nucleotidesPFLU_RS21120 and PFLU_RS21125 are separated by 100 nucleotidesPFLU_RS21125 and PFLU_RS21130 are separated by 302 nucleotides PFLU_RS21110: PFLU_RS21110 - AAA family ATPase, at 4,744,987 to 4,745,796 _RS21110 PFLU_RS21115: PFLU_RS21115 - tRNA-Asn, at 4,745,918 to 4,745,993 _RS21115 PFLU_RS21120: PFLU_RS21120 - LysR family transcriptional regulator, at 4,746,054 to 4,746,950 _RS21120 PFLU_RS21125: PFLU_RS21125 - FMN-dependent L-lactate dehydrogenase LldD, at 4,747,051 to 4,748,193 _RS21125 PFLU_RS21130: PFLU_RS21130 - GGDEF domain-containing protein, at 4,748,496 to 4,749,422 _RS21130
Group Condition PFLU_RS21110 PFLU_RS21115 PFLU_RS21120 PFLU_RS21125 PFLU_RS21130
carbon source L-Lactate (C) +0.4 N.D. -3.0 -4.5 +0.3
carbon source L-Lactate 20 mM (C) +0.0 N.D. -2.7 -3.9 -0.2
carbon source L-Lactate (C) -0.4 N.D. -2.4 -3.9 -0.0
carbon source L-Lactate 20 mM (C) -0.2 N.D. -2.4 -3.9 +0.0
carbon source L-Lactate 20 mM (C) -0.4 N.D. -2.1 -4.0 +0.1
carbon source L-Lactate (C) +0.6 N.D. -2.8 -4.1 +0.1
temperature Growth at 25C -2.3 N.D. -0.6 -0.2 -0.0
carbon source D-Glucosamine Hydrochloride 10 mM (C) -3.3 N.D. +0.1 +0.2 +0.1
carbon source L-Carnitine (C) -3.0 N.D. -0.5 +0.3 +0.4
carbon source 4-Acetoxy-3-methoxycinnamic 2 mM (C) -0.8 N.D. -0.9 -0.3 -0.6
temperature shift Temperature shift 10_to_25; with MOPS -2.1 N.D. -0.0 -0.0 -0.3
carbon source propionate 20 mM (C) -1.2 N.D. -1.4 +0.1 +0.2
carbon source Ferulic Acid 2 mM (C) -1.1 N.D. -0.6 -0.1 -0.5
no stress control Glycine betaine (C)(N); with TAPS -1.8 N.D. -0.2 +0.1 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -2.0 N.D. +0.1 -0.2 +0.2
carbon source D,L-Lactate 20 mM (C) -0.4 N.D. -0.7 -0.9 +0.2
pH Growth at pH8 and (C) D-Glucose -1.7 N.D. -0.2 -0.2 +0.3
carbon source NAG (C) -2.2 N.D. +0.5 -0.1 -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -1.6 N.D. +0.1 +0.1 -0.4
no stress control Growth with no stress -1.9 N.D. +0.2 +0.0 +0.1
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -1.8 N.D. -0.2 +0.2 +0.2
carbon source D,L-Lactate 20 mM (C) +0.6 N.D. -1.0 -0.9 -0.0
carbon source Xanthosine (C) -0.8 N.D. -1.2 +0.1 +0.6
carbon source p-Coumaric 2 mM (C) -1.0 N.D. -0.7 +0.1 +0.4
carbon source Phloretic Acid 2 mM (C) +0.3 N.D. -1.5 +0.3 +0.2
carbon source propionate 20 mM (C) +0.5 N.D. -1.0 +0.4 -0.5
stress Betaine (C)(N); with MOPS; with chloride +1.2 N.D. -0.5 +0.3 -0.5
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +1.1 N.D. -0.8 +0.4 -0.1
carbon source Xanthosine (C) +0.8 N.D. -0.8 +0.4 +0.8
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +1.6 N.D. -0.3 +0.2 +0.5
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