Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS30970 and PFLU_RS31710 are separated by 692 nucleotidesPFLU_RS31710 and PFLU_RS20225 are separated by 679 nucleotidesPFLU_RS20225 and PFLU_RS30975 are separated by 100 nucleotidesPFLU_RS30975 and PFLU_RS20235 are separated by 186 nucleotides PFLU_RS30970: PFLU_RS30970 - hypothetical protein, at 4,574,483 to 4,574,743 _RS30970 PFLU_RS31710: PFLU_RS31710 - hypothetical protein, at 4,575,436 to 4,575,735 _RS31710 PFLU_RS20225: PFLU_RS20225 - glycoside hydrolase family 19 protein, at 4,576,415 to 4,576,948 _RS20225 PFLU_RS30975: PFLU_RS30975 - acyltransferase, at 4,577,049 to 4,578,965 _RS30975 PFLU_RS20235: PFLU_RS20235 - tail fiber assembly protein, at 4,579,152 to 4,579,496 _RS20235
Group Condition PFLU_RS30970 PFLU_RS31710 PFLU_RS20225 PFLU_RS30975 PFLU_RS20235
no stress control L-Glutamine (C)(N); with TAPS -0.0 -4.5 +0.2 -0.2 +0.0
carbon source 4-Acetoxy-3-methoxycinnamic 2 mM (C) -0.0 -4.3 -0.0 +0.3 -0.4
carbon source L-Valine (C) -0.7 -3.5 -0.5 +0.1 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.6 -2.8 +0.0 -0.4 +0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.3 -2.7 +0.0 -0.3 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.0 -2.5 +0.1 +0.2 -0.0
pH Growth at pH8 and (C) Trisodium citrate -0.3 -2.4 -0.3 -0.4 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.7 -1.7 +0.2 -0.4 -0.3
stress Betaine (C)(N); with MOPS; with chloride -0.7 -1.5 -0.8 +0.1 +0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.3 -0.9 -0.5 -0.3 -0.6
stress Betaine (C)(N); with TAPS; with chloride -0.0 -2.5 +0.2 -0.0 -0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.7 -2.0 +0.4 -0.0 -0.3
carbon source Quinic Acid (C) -0.1 -2.5 +0.2 +0.0 -0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.3 -1.5 +0.4 -0.0 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.3 -2.4 -0.3 +0.0 -0.1
stress Betaine (C)(N); with MOPS; with chloride +0.3 -2.7 -0.1 -0.1 +0.1
carbon source L-Carnitine (C) -0.7 -1.8 -0.3 +0.2 +0.2
carbon source L-Ornithine (C) -0.2 -3.0 +0.3 +0.2 +0.4
temperature Growth at 25C -0.2 -1.6 -0.7 +0.1 -0.1
stress Betaine (C)(N); with TAPS; with chloride +0.1 -2.1 -0.3 -0.2 +0.3
carbon source D-Glucose (C); with MOPS -0.2 -2.1 +0.2 +0.2 -0.2
carbon source D-Glucose (C) -0.6 -1.8 +0.1 +0.4 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.6 -1.5 +0.3 +0.4 -0.5
no stress control L-Glutamine (C)(N); with TAPS +0.1 -2.1 +0.2 +0.1 -0.3
no stress control Glycine betaine (C)(N); with MOPS -0.3 -2.0 +0.4 +0.3 -0.4
carbon source 4-Aminobutyric (C) -1.2 -0.1 +0.3 +0.4 -0.9
carbon source Xanthosine (C) +0.3 -2.2 +0.3 +0.1 +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.2 +1.3 -0.4 -0.2 -0.1
stress malate (C) and Ammonium chloride (N); with MOPS; with PEG +0.1 -0.8 -0.2 +1.6 -0.0
stress malate (C) and Ammonium chloride (N); with MOPS; with PEG +0.1 -0.3 +0.2 +2.4 +0.0
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