Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS17980 and PFLU_RS17990 are separated by 841 nucleotidesPFLU_RS17990 and PFLU_RS17995 are separated by 12 nucleotidesPFLU_RS17995 and PFLU_RS18000 overlap by 4 nucleotidesPFLU_RS18000 and PFLU_RS18005 are separated by 30 nucleotides PFLU_RS17980: PFLU_RS17980 - sulfurtransferase, at 4,079,879 to 4,081,462 _RS17980 PFLU_RS17990: PFLU_RS17990 - ABC transporter substrate-binding protein, at 4,082,304 to 4,083,278 _RS17990 PFLU_RS17995: PFLU_RS17995 - LLM class flavin-dependent oxidoreductase, at 4,083,291 to 4,084,655 _RS17995 PFLU_RS18000: PFLU_RS18000 - AhpC/TSA family protein, at 4,084,652 to 4,085,335 _RS18000 PFLU_RS18005: PFLU_RS18005 - acyl-CoA/acyl-ACP dehydrogenase, at 4,085,366 to 4,086,544 _RS18005
Group Condition PFLU_RS17980 PFLU_RS17990 PFLU_RS17995 PFLU_RS18000 PFLU_RS18005
carbon source Phloretic Acid 2 mM (C) +0.1 -3.0 +0.4 -3.1 -1.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 -1.7 -0.3 -0.8 -3.6
stress Betaine (C)(N); with MOPS; with chloride +0.4 -0.5 -0.1 -1.0 -4.6
temperature Growth at 25C; with MOPS -0.4 -0.4 -0.3 -3.1 -1.1
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.0 -0.8 -0.3 -0.6 -3.2
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS +0.0 -0.4 -0.3 -4.3 +0.1
stress Betaine (C)(N); with TAPS; with chloride +0.1 -0.0 +0.3 -0.4 -4.4
pH Growth at pH6 and (C) D-Glucose -0.2 -0.0 -0.1 -1.3 -2.5
carbon source propionate 20 mM (C) +0.1 -0.8 -0.2 -1.4 -1.7
stress Betaine (C)(N); with MOPS; with chloride +0.3 -0.0 -0.1 -4.0 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.0 +0.3 -1.1 -1.7 -1.2
no stress control Glycine betaine (C)(N); with TAPS -0.3 -0.5 +0.6 -2.9 -0.6
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS +0.1 -0.5 +0.4 -0.5 -3.0
carbon source L-Lysine (C) -0.1 +0.1 +0.2 -3.0 -0.6
stress Betaine (C)(N); with MOPS; with chloride +0.2 +0.1 +0.3 -2.8 -1.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.2 +0.4 -0.4 -1.1 -2.2
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.0 -1.7 +0.2 +0.5 -2.1
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.3 +0.4 -0.8 -0.6 -2.2
carbon source L-Valine (C) -0.1 -1.2 -0.6 -1.5 +0.8
nitrogen source NAG (N); with MOPS -0.4 +0.6 +0.5 -2.7 -0.6
stress Betaine (C)(N); with MOPS; with chloride +0.6 +0.2 +0.0 -3.3 +0.0
carbon source caffeic 2 mM (C) +0.2 -0.2 -0.0 -3.0 +0.6
carbon source 4-Aminobutyric (C) +0.4 +0.8 +0.1 -2.3 -0.8
stress Betaine (C)(N); with MOPS; with chloride +0.3 +0.4 +0.4 -3.2 +0.6
pH Growth at pH9 and (C) Trisodium citrate -0.3 -1.3 +0.8 -1.3 +0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.1 +1.0 +0.1 +0.2 -2.7
carbon source Inosine (C) +0.0 -0.1 +0.4 +2.1 -1.8
carbon source D-Glucuronic (C) +0.4 -0.4 +0.5 +1.8 -1.5
carbon source 4-Aminobutyric (C) -0.4 +1.1 +0.5 +1.1 -1.5
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.3 +1.1 -0.5 +1.4 +0.8
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