Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS17945 and PFLU_RS17950 overlap by 4 nucleotidesPFLU_RS17950 and PFLU_RS17955 overlap by 49 nucleotidesPFLU_RS17955 and PFLU_RS17960 are separated by 287 nucleotidesPFLU_RS17960 and PFLU_RS17965 are separated by 243 nucleotides PFLU_RS17945: PFLU_RS17945 - hypothetical protein, at 4,074,519 to 4,074,728 _RS17945 PFLU_RS17950: PFLU_RS17950 - hypothetical protein, at 4,074,725 to 4,075,057 _RS17950 PFLU_RS17955: PFLU_RS17955 - hypothetical protein, at 4,075,009 to 4,075,632 _RS17955 PFLU_RS17960: PFLU_RS17960 - LLM class flavin-dependent oxidoreductase, at 4,075,920 to 4,077,002 _RS17960 PFLU_RS17965: PFLU_RS17965 - class II aldolase/adducin family protein, at 4,077,246 to 4,078,040 _RS17965
Group Condition PFLU_RS17945 PFLU_RS17950 PFLU_RS17955 PFLU_RS17960 PFLU_RS17965
nitrogen source nitrate (N); with MOPS N.D. -4.3 -0.3 -0.1 -1.0
temperature Growth at 25C; with MOPS N.D. -4.4 -0.9 -0.1 -0.3
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride N.D. -3.7 -0.0 -0.3 -0.5
stress Glucose (C) and Betaine (N); with TAPS; with chloride N.D. -4.2 -0.1 -0.2 +0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride N.D. -3.4 -0.0 -0.0 -0.9
pH Growth at pH9 and (C) Trisodium citrate N.D. -3.8 -0.8 -0.1 +0.3
carbon source L-Carnitine (C) N.D. -3.0 +0.1 -0.1 -1.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. -3.5 -0.8 -0.0 +0.2
stress Betaine (C)(N); with TAPS; with chloride N.D. -3.2 +0.3 -0.0 -1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -3.7 -0.4 -0.1 +0.4
carbon source D-Mannitol (C) N.D. -2.7 -0.4 -0.2 -0.5
stress Betaine (C)(N); with MOPS; with chloride N.D. -3.1 -1.1 +0.5 +0.0
stress Betaine (C)(N); with MOPS; with chloride N.D. -2.7 -0.6 +0.4 -0.6
stress Betaine (C)(N); with TAPS; with chloride N.D. -2.8 -0.8 -0.5 +0.6
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride N.D. -2.1 -1.2 -0.2 +0.1
pH Growth at pH8 and (C) D-Glucose N.D. -3.2 +0.1 -0.4 +0.2
carbon source acetate (C) N.D. -3.2 -0.2 -0.1 +0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride N.D. -3.0 -0.3 +0.1 -0.2
stress Glucose (C) and L-Glutamine (N); with TAPS; with chloride N.D. -3.1 -0.1 +0.1 -0.1
nitrogen source no extra (N); with MOPS N.D. -2.7 -0.4 -0.3 +0.1
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS N.D. -2.9 -0.3 -0.3 +0.7
carbon source octanoate 10 mM (C) N.D. -3.0 +0.3 +0.2 -0.2
stress Glucose (C) and L-Glutamine (N); with MOPS; with chloride N.D. -3.0 +0.3 -0.2 +0.3
carbon source L-Valine (C) N.D. -2.8 -0.6 +0.2 +0.5
no stress control Glucose (C) and nitrate (N); with MOPS N.D. -3.1 -0.1 +0.3 +0.2
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS N.D. -2.0 -1.0 +0.2 +0.4
carbon source m-Inositol (C) N.D. -2.3 +0.8 -0.3 -0.3
carbon source L-Carnitine (C) N.D. -2.3 +0.2 +0.6 -0.5
no stress control Glucose (C) and Betaine (N); with MOPS N.D. -2.4 +0.5 +0.4 -0.1
stress Betaine (C)(N); with MOPS; with chloride N.D. -2.4 +0.2 +0.2 +0.6
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