Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS17255 and PFLU_RS17260 overlap by 4 nucleotidesPFLU_RS17260 and PFLU_RS17265 are separated by 253 nucleotidesPFLU_RS17265 and PFLU_RS17270 are separated by 4 nucleotidesPFLU_RS17270 and PFLU_RS17275 overlap by 4 nucleotides PFLU_RS17255: PFLU_RS17255 - metal-dependent hydrolase, at 3,924,935 to 3,925,768 _RS17255 PFLU_RS17260: PFLU_RS17260 - PTS sugar transporter subunit IIC/EAL domain-containing protein, at 3,925,765 to 3,927,840 _RS17260 PFLU_RS17265: PFLU_RS17265 - hypothetical protein, at 3,928,094 to 3,928,423 _RS17265 PFLU_RS17270: PFLU_RS17270 - serine/threonine protein kinase, at 3,928,428 to 3,929,273 _RS17270 PFLU_RS17275: PFLU_RS17275 - serine/threonine-protein phosphatase, at 3,929,270 to 3,930,013 _RS17275
Group Condition PFLU_RS17255 PFLU_RS17260 PFLU_RS17265 PFLU_RS17270 PFLU_RS17275
carbon source NAG (C) +0.3 -0.7 -3.6 -0.8 -0.8
carbon source Phloretic Acid 2 mM (C) -0.5 +0.1 -4.4 -0.3 -0.1
carbon and nitrogen source NAG carbon and (N) +0.0 -1.0 -3.6 -0.7 +0.1
nitrogen source nitrate (N); with MOPS +0.1 +0.0 -4.5 -0.3 -0.2
stress Betaine (C)(N); with MOPS; with chloride -0.0 +0.1 -4.6 +0.1 -0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.0 -0.0 -3.9 -0.5 -0.4
carbon source Phloretic Acid 2 mM (C) +0.3 -0.2 -4.4 -0.1 -0.3
pH Growth at pH7 and (C) D-Glucose +0.1 -0.2 -4.6 +0.1 +0.1
carbon and nitrogen source NAG carbon and (N) +0.1 -1.1 -1.7 -1.0 -0.6
carbon source 4-Aminobutyric (C) -0.5 -0.0 -1.8 -0.8 -1.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -1.7 -0.2 -1.8 -0.2 -0.2
carbon source NAG (C) -0.7 -0.7 -1.0 -1.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 +0.5 -4.6 -0.0 +0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.8 -0.5 -0.8 -1.3 -0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.5 -0.1 -1.7 -1.0 -0.4
nitrogen source NAG (N); with MOPS +0.5 +0.0 -4.2 +0.0 -0.0
no stress control Glycine betaine (C)(N); with TAPS -0.7 -0.2 -2.4 -0.1 -0.3
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.1 +0.0 -3.3 -0.3 +0.2
carbon source NAG (C) +0.1 -0.5 -1.6 -1.3 -0.1
carbon source L-Arabinose (C) +0.2 -0.1 -3.1 -0.3 -0.1
stress Betaine (C)(N); with MOPS; with chloride +0.3 +0.1 -2.6 -0.5 -0.6
carbon source 4-hydroxyphenylacetic 2 mM (C) -0.1 -0.0 -2.8 +0.5 -0.1
stress Betaine (C)(N); with TAPS; with chloride +0.4 -0.4 -4.3 +1.2 +0.6
nitrogen source NAG (N); with MOPS +0.4 -0.5 -2.2 +0.3 -0.4
stress Betaine (C)(N); with TAPS; with chloride -0.0 -0.6 -3.2 +0.9 +0.7
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS +0.2 +0.1 -3.0 +0.4 +0.7
carbon source D-Glucosamine Hydrochloride 10 mM (C) +0.2 -0.1 -2.3 +0.5 +0.4
carbon source caffeic 2 mM (C) +1.1 +0.1 -1.5 -0.5 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.2 -0.1 +1.8 +1.1 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.5 -0.1 +2.0 +1.1 +0.2
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