Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS17180 and PFLU_RS31680 are separated by 132 nucleotidesPFLU_RS31680 and PFLU_RS17185 are separated by 129 nucleotidesPFLU_RS17185 and PFLU_RS17190 are separated by 31 nucleotidesPFLU_RS17190 and PFLU_RS17195 overlap by 4 nucleotides PFLU_RS17180: PFLU_RS17180 - Gfo/Idh/MocA family oxidoreductase, at 3,904,709 to 3,905,824 _RS17180 PFLU_RS31680: PFLU_RS31680 - gfo/Idh/MocA family oxidoreductase, at 3,905,957 to 3,906,088 _RS31680 PFLU_RS17185: PFLU_RS17185 - AMP-binding protein, at 3,906,218 to 3,907,921 _RS17185 PFLU_RS17190: PFLU_RS17190 - acetyl-CoA C-acetyltransferase, at 3,907,953 to 3,909,251 _RS17190 PFLU_RS17195: PFLU_RS17195 - acyl dehydratase, at 3,909,248 to 3,910,138 _RS17195
Group Condition PFLU_RS17180 PFLU_RS31680 PFLU_RS17185 PFLU_RS17190 PFLU_RS17195
pH Growth at pH8 and (C) Trisodium citrate -0.3 N.D. -0.7 -0.9 -1.2
stress L-Glutamine (C)(N); with MOPS; with chloride -0.0 N.D. -0.2 -0.5 -2.0
carbon source p-Coumaric (C) 5 mM -0.5 N.D. -0.3 -0.2 -1.8
carbon source L-Ornithine 10 mM (C) -0.1 N.D. +0.1 -0.5 -2.1
pH Growth at pH9 and (C) Trisodium citrate +0.1 N.D. -0.7 -1.3 -0.7
carbon source Shikimic Acid (C) -1.2 N.D. +0.4 +0.5 -2.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.0 N.D. -0.5 -1.6 -0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.4 N.D. -0.4 -0.1 -1.5
stress Betaine (C)(N); with MOPS; with chloride +0.4 N.D. -0.4 -1.1 -1.1
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS +0.4 N.D. -0.8 -0.7 -1.1
carbon source L-Valine (C) -0.7 N.D. -0.6 -0.8 -0.1
carbon source Sucrose 5 mM (C) -0.3 N.D. +0.1 -0.3 -1.6
carbon source Trisodium citrate (C) -0.1 N.D. +0.2 -0.2 -1.9
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 N.D. +0.1 -1.4 -1.0
carbon source L-Carnitine (C) +0.3 N.D. +0.1 +0.2 -2.4
carbon source 4-Aminobutyric (C) +0.4 N.D. -0.8 +0.2 -1.6
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.4 N.D. -0.1 -0.2 -1.7
stress L-Glutamine (C)(N); with TAPS; with chloride +0.2 N.D. -0.6 -1.5 +0.2
no stress control Glucose (C) and Ammonium chloride (N); with TAPS +0.2 N.D. +0.1 +0.0 -1.8
no stress control L-Glutamine (C)(N); with TAPS +0.5 N.D. -0.5 -0.2 -1.1
stress Betaine (C)(N); with TAPS; with chloride +0.3 N.D. +0.4 -0.3 -1.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.8 N.D. -0.6 -0.1 -1.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.5 N.D. +0.2 -1.1 +0.4
carbon source Phloretic Acid 2 mM (C) +0.6 N.D. +0.2 -0.6 -0.8
carbon source p-Coumaric 2 mM (C) +0.8 N.D. -0.9 -0.1 +0.5
carbon source D,L-Malic Acid (C) +0.2 N.D. -0.2 -0.7 +1.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.6 N.D. -0.3 -0.9 +1.1
stress Betaine (C)(N); with MOPS; with chloride -0.2 N.D. +0.4 -0.7 +1.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.2 N.D. -0.4 +1.2 +0.6
no stress control L-Glutamine (C)(N); with MOPS +0.6 N.D. +0.1 +0.5 +1.1
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