Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS17035 and PFLU_RS17040 are separated by 129 nucleotidesPFLU_RS17040 and PFLU_RS17045 are separated by 22 nucleotidesPFLU_RS17045 and PFLU_RS17050 are separated by 0 nucleotidesPFLU_RS17050 and PFLU_RS17055 are separated by 155 nucleotides PFLU_RS17035: PFLU_RS17035 - 2-hydroxyacid dehydrogenase, at 3,863,801 to 3,864,754 _RS17035 PFLU_RS17040: PFLU_RS17040 - phosphotransferase family protein, at 3,864,884 to 3,865,951 _RS17040 PFLU_RS17045: PFLU_RS17045 - SDR family oxidoreductase, at 3,865,974 to 3,866,741 _RS17045 PFLU_RS17050: PFLU_RS17050 - carboxymuconolactone decarboxylase family protein, at 3,866,742 to 3,867,185 _RS17050 PFLU_RS17055: PFLU_RS17055 - PLP-dependent aminotransferase family protein, at 3,867,341 to 3,868,774 _RS17055
Group Condition PFLU_RS17035 PFLU_RS17040 PFLU_RS17045 PFLU_RS17050 PFLU_RS17055
carbon source L-Isoleucine (C) -0.6 +0.0 -2.2 N.D. -0.0
stress Glucose (C) and L-Glutamine (N); with MOPS; with chloride -0.3 -0.1 -1.0 N.D. -0.2
no stress control Glycine betaine (C)(N); with MOPS -1.0 -0.4 -0.0 N.D. -0.1
carbon source sn-glycero-3-phosphocholine 10 mM (C) -0.3 -0.1 -1.2 N.D. +0.0
nitrogen source nitrate (N); with MOPS -0.0 -0.2 -1.4 N.D. +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.1 -0.1 -1.4 N.D. +0.1
nitrogen source NAG (N); with MOPS +0.1 -0.4 -1.0 N.D. +0.1
stress Betaine (C)(N); with MOPS; with chloride +0.2 +0.1 -1.6 N.D. +0.1
carbon source Shikimic Acid (C) -0.2 +0.5 -0.9 N.D. -0.6
carbon source 2-Keto-D-gluconic hemicalcium salt (C) -0.4 -0.5 -0.2 N.D. +0.3
stress malate (C) and Ammonium chloride (N); with TAPS +0.4 -0.2 -1.0 N.D. -0.2
carbon source Ferulic Acid (C) 1.5 mM +0.2 +0.1 -1.3 N.D. +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.6 -0.0 +0.5 N.D. -0.3
carbon source D-(+)-Galactosamine 10 mM (C) +0.3 -0.0 -1.0 N.D. +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.6 +0.1 -0.9 N.D. -0.1
no stress control Glycine betaine (C)(N); with TAPS -1.0 +0.0 +0.9 N.D. -0.2
carbon source D-Galactose (C) +0.5 +0.1 -0.8 N.D. +0.0
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.7 -0.2 -0.9 N.D. +0.4
carbon source Phloretic Acid 2 mM (C) -1.0 +0.5 +0.4 N.D. +0.2
carbon source 4-Guanidinobutyric (C) +0.6 +0.2 -0.7 N.D. +0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 -0.2 +0.9 N.D. -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +1.0 -0.1 -0.1 N.D. +0.4
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.0 +0.3 +1.2 N.D. -0.3
carbon source Xanthosine (C) +0.3 -0.1 +1.0 N.D. +0.1
temperature Growth at 25C; with MOPS +0.4 +0.0 +0.9 N.D. +0.2
stress Betaine (C)(N); with MOPS; with chloride +0.4 +0.1 +0.8 N.D. +0.3
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.5 -0.1 +0.8 N.D. +0.3
carbon source L-Lysine (C) +0.8 -0.1 +0.6 N.D. +0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.8 +0.5 +0.3 N.D. +0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.6 +0.4 +0.5 N.D. +0.5
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