Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS16875 and PFLU_RS16880 are separated by 23 nucleotidesPFLU_RS16880 and PFLU_RS16885 are separated by 92 nucleotidesPFLU_RS16885 and PFLU_RS16890 overlap by 4 nucleotidesPFLU_RS16890 and PFLU_RS16895 are separated by 134 nucleotides PFLU_RS16875: PFLU_RS16875 - nuclear transport factor 2 family protein, at 3,827,313 to 3,827,741 _RS16875 PFLU_RS16880: PFLU_RS16880 - PLP-dependent aminotransferase family protein, at 3,827,765 to 3,829,090 _RS16880 PFLU_RS16885: PFLU_RS16885 - DMT family transporter, at 3,829,183 to 3,830,073 _RS16885 PFLU_RS16890: PFLU_RS16890 - YceI family protein, at 3,830,070 to 3,830,624 _RS16890 PFLU_RS16895: PFLU_RS16895 - thioesterase, at 3,830,759 to 3,831,253 _RS16895
Group Condition PFLU_RS16875 PFLU_RS16880 PFLU_RS16885 PFLU_RS16890 PFLU_RS16895
carbon source Xanthosine (C) +0.0 -0.3 -0.5 +0.0 -1.8
carbon source Quinic Acid (C) -0.2 +0.2 -0.9 -0.7 -0.4
no stress control L-Glutamine (C)(N); with MOPS -0.6 -0.2 -0.8 +0.2 -0.4
no stress control L-Glutamine (C)(N); with TAPS -0.1 -1.2 -0.3 +0.4 -0.5
carbon source Inosine (C) +0.1 +0.7 -0.0 +0.4 -2.6
carbon source Inosine (C) -0.1 +0.4 -0.5 -0.7 -0.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.7 +0.2 -0.5 +0.3 -0.6
carbon source L-Carnitine (C) +0.5 +0.7 -1.1 -0.0 -1.3
carbon source Ferulic Acid (C) 1.5 mM +0.0 +0.2 -1.1 +0.4 -0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 -0.2 +1.0 -0.7 -1.7
carbon source p-Coumaric (C) 5 mM -0.2 -0.0 +0.7 -0.3 -0.9
pH Growth at pH8 and (C) D-Glucose -0.2 +0.1 -0.8 -0.5 +0.8
stress Betaine (C)(N); with MOPS; with chloride -1.1 +0.1 +0.6 +0.3 -0.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.6 -0.3 +0.5 -0.6 -0.9
carbon source Xanthosine (C) -0.3 +0.5 -0.1 +0.4 -1.0
carbon source L-Valine (C) -1.0 -0.1 +1.1 -0.0 -0.4
carbon source Shikimic Acid (C) +0.3 +0.2 -1.2 +0.4 -0.1
stress Betaine (C)(N); with MOPS; with chloride +0.5 -0.4 +0.7 -0.1 -0.6
carbon source 2-Deoxy-D-Ribose 10 mM (C) +0.6 -0.5 +0.7 -0.1 -0.7
stress Betaine (C)(N); with MOPS; with chloride +0.2 +0.2 +0.9 -0.9 -0.3
carbon source L-Carnitine (C) +0.2 +0.4 -0.8 -0.4 +0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.9 +0.2 +0.4 -0.3 -1.0
nitrogen source no extra (N); with MOPS -0.1 -0.3 +0.5 -0.5 +0.9
carbon source L-Lysine (C) -1.0 -0.1 +0.7 +0.3 +0.8
carbon source caffeic 2 mM (C) +0.2 -0.5 +0.3 -0.2 +1.1
carbon source L-Valine 10 mM (C) -0.1 -0.5 +0.2 -0.1 +1.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.2 +0.6 -0.6 +0.4 +0.8
carbon source L-Valine 10 mM (C) +0.3 -0.0 +1.1 -0.3 +0.4
stress Betaine (C)(N); with MOPS; with chloride +0.2 +0.6 +0.5 -0.3 +0.8
carbon source L-Valine 10 mM (C) +0.1 -0.1 +1.0 -0.1 +1.2
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