Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS16645 and PFLU_RS16650 are separated by 186 nucleotidesPFLU_RS16650 and PFLU_RS16655 are separated by 41 nucleotidesPFLU_RS16655 and PFLU_RS16660 are separated by 48 nucleotidesPFLU_RS16660 and PFLU_RS16665 are separated by 56 nucleotides PFLU_RS16645: PFLU_RS16645 - GNAT family N-acetyltransferase, at 3,774,578 to 3,775,054 _RS16645 PFLU_RS16650: PFLU_RS16650 - PD-(D/E)XK nuclease family protein, at 3,775,241 to 3,776,518 _RS16650 PFLU_RS16655: PFLU_RS16655 - hypothetical protein, at 3,776,560 to 3,777,393 _RS16655 PFLU_RS16660: PFLU_RS16660 - hypothetical protein, at 3,777,442 to 3,778,167 _RS16660 PFLU_RS16665: PFLU_RS16665 - cysteine hydrolase, at 3,778,224 to 3,778,784 _RS16665
Group Condition PFLU_RS16645 PFLU_RS16650 PFLU_RS16655 PFLU_RS16660 PFLU_RS16665
carbon source Xanthosine (C) -0.1 -0.6 -1.1 -1.3 -0.7
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.6 +0.0 -1.1 -0.8 -0.9
pH Growth at pH8 and (C) Trisodium citrate -0.6 +0.0 -0.9 +0.0 -1.8
carbon source L-Carnitine (C) +0.1 -0.6 -0.2 -0.8 -1.5
carbon source Xanthosine (C) -0.3 +0.2 -1.8 -1.0 -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.2 +0.2 -1.4 -0.3 -1.0
stress L-Glutamine (C)(N); with MOPS; with chloride +0.3 -0.3 -0.9 -0.5 -1.2
carbon source D,L-Malic Acid (C) +0.0 +0.2 -0.7 -0.6 -1.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.1 -0.1 +0.7 -1.5 -1.6
stress Betaine (C)(N); with MOPS; with chloride +0.1 -0.3 -1.2 -0.3 -0.6
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.2 +0.0 -0.9 +0.1 -1.7
stress L-Glutamine (C)(N); with TAPS; with chloride -0.4 -0.3 -0.4 -1.5 +0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.7 +0.1 -1.2 -1.0 +0.7
stress Betaine (C)(N); with MOPS; with chloride -0.0 -0.2 -1.9 +0.3 -0.2
nitrogen source no extra (N); with MOPS +0.2 -0.2 -0.3 -0.6 -1.2
carbon source D-(+)-Galactosamine (C) -0.1 -0.4 -0.5 +0.4 -1.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.3 +0.1 -2.0 -0.1 +0.3
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS -0.4 +0.3 -0.1 -0.7 -1.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.6 -0.1 -0.8 -0.9 +0.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.3 +0.1 -1.5 -0.1 -0.5
temperature Growth at 25C +0.4 -0.2 -1.1 -1.0 +0.5
nitrogen source nitrate (N); with MOPS -0.5 -0.5 +0.5 -0.1 -0.9
carbon source Xanthosine (C) -0.3 +0.4 -0.7 -0.7 +0.4
carbon source L-Ornithine 10 mM (C) +0.1 +0.2 -1.0 +0.5 -0.6
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.7 -0.1 +0.2 -0.5 +0.8
carbon source L-Valine 10 mM (C) +0.8 -0.0 -0.3 +0.5 -0.6
carbon source L-Valine (C) -0.8 -0.4 -0.2 +1.8 -0.0
stress Betaine (C)(N); with MOPS; with chloride +0.1 +0.2 -0.9 +0.7 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.9 +0.3 +0.3 +0.1 +1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.6 -0.2 +0.3 -0.6 +1.2
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