Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS15990 and PFLU_RS31665 are separated by 400 nucleotidesPFLU_RS31665 and PFLU_RS15995 are separated by 33 nucleotidesPFLU_RS15995 and PFLU_RS16000 are separated by 14 nucleotidesPFLU_RS16000 and PFLU_RS16005 are separated by 116 nucleotides PFLU_RS15990: PFLU_RS15990 - SDR family oxidoreductase, at 3,631,315 to 3,632,085 _RS15990 PFLU_RS31665: PFLU_RS31665 - hypothetical protein, at 3,632,486 to 3,632,674 _RS31665 PFLU_RS15995: PFLU_RS15995 - TetR/AcrR family transcriptional regulator, at 3,632,708 to 3,633,292 _RS15995 PFLU_RS16000: PFLU_RS16000 - transcriptional regulator FeaR, at 3,633,307 to 3,634,269 _RS16000 PFLU_RS16005: PFLU_RS16005 - DUF3156 family protein, at 3,634,386 to 3,635,006 _RS16005
Group Condition PFLU_RS15990 PFLU_RS31665 PFLU_RS15995 PFLU_RS16000 PFLU_RS16005
carbon source Choline chloride 10 mM (C) -4.8 N.D. +0.2 +0.3 -2.4
carbon source acetate 30 mM (C) -3.9 N.D. -0.6 +0.1 -1.5
carbon source 2-Deoxy-D-Ribose 10 mM (C) -2.1 N.D. -0.8 -0.9 -1.9
carbon source p-Coumaric 2 mM (C) -3.7 N.D. +0.2 +0.5 -1.4
carbon source Inosine (C) -2.7 N.D. -0.7 -0.4 +0.0
carbon source 4-Acetoxy-3-methoxycinnamic 2 mM (C) -3.5 N.D. +0.3 -0.2 -0.1
carbon source 4-Acetoxy-3-methoxycinnamic 2 mM (C) -3.8 N.D. +0.3 -0.0 +0.1
carbon source succinate (C); with MOPS -3.1 N.D. -0.3 +0.0 -0.0
no stress control Glucose (C) and nitrate (N); with TAPS -3.0 N.D. +0.2 +0.2 -0.7
pH Growth at pH9 and (C) D-Glucose -1.7 N.D. -0.8 -0.5 -0.3
carbon source NAG (C) -1.2 N.D. -2.4 +0.0 +0.3
stress Sucrose (C) and L-Glutamine (N); with MOPS; with PEG -3.4 N.D. +0.7 -0.0 -0.4
pH Growth at pH9 and (C) Trisodium citrate -3.5 N.D. -0.0 -0.2 +0.6
nitrogen source nitrate (N); with MOPS -3.1 N.D. -0.1 -0.6 +0.8
carbon source L-Lysine (C) +0.6 N.D. -0.7 -0.5 -1.9
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -2.8 N.D. -0.1 +0.4 -0.0
carbon source Xanthosine (C) -3.8 N.D. +0.6 +0.6 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.7 N.D. +0.4 +0.3 -1.3
no stress control L-Glutamine (C)(N); with MOPS -0.7 N.D. +0.5 -0.3 -1.8
carbon source L-Valine (C) -3.1 N.D. +0.5 +0.3 -0.0
pH Growth at pH9 and (C) Trisodium citrate -1.5 N.D. +0.1 -1.2 +0.8
carbon source 4-hydroxyphenylacetic 2 mM (C) -1.7 N.D. +0.4 +0.4 -0.9
no stress control Glycine betaine (C)(N); with TAPS -4.0 N.D. +0.9 +0.7 +0.6
stress Betaine (C)(N); with TAPS; with chloride +1.0 N.D. -0.3 -0.2 -1.8
carbon source L-Carnitine (C) -1.8 N.D. -0.4 +0.7 +0.7
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.7 N.D. +0.4 +0.6 +0.6
stress Betaine (C)(N); with MOPS; with chloride +1.4 N.D. +0.5 -1.3 +0.2
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +1.7 N.D. -0.7 +0.5 -0.7
carbon source Shikimic Acid (C) +2.2 N.D. +0.2 -0.4 -1.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +1.5 N.D. -1.1 -0.4 +1.2
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