Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS15920 and PFLU_RS15925 overlap by 4 nucleotidesPFLU_RS15925 and PFLU_RS15930 are separated by 5 nucleotidesPFLU_RS15930 and PFLU_RS15935 are separated by 50 nucleotidesPFLU_RS15935 and PFLU_RS15940 are separated by 45 nucleotides PFLU_RS15920: PFLU_RS15920 - 4-hydroxyphenylacetate isomerase, at 3,618,884 to 3,619,552 _RS15920 PFLU_RS15925: PFLU_RS15925 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 3,619,549 to 3,620,319 _RS15925 PFLU_RS15930: PFLU_RS15930 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 3,620,325 to 3,621,785 _RS15930 PFLU_RS15935: PFLU_RS15935 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 3,621,836 to 3,622,693 _RS15935 PFLU_RS15940: PFLU_RS15940 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 3,622,739 to 3,623,131 _RS15940
Group Condition PFLU_RS15920 PFLU_RS15925 PFLU_RS15930 PFLU_RS15935 PFLU_RS15940
carbon source 4-hydroxyphenylacetic 2 mM (C) -4.4 -4.4 -4.5 -4.2 N.D.
carbon source 4-hydroxyphenylacetic 2 mM (C) -4.1 -4.7 -4.5 -3.9 N.D.
carbon source 4-hydroxyphenylacetic 2 mM (C) -4.9 -4.6 -4.1 -3.5 N.D.
carbon source L-tyrosine 10 mM (C) -1.1 -1.1 -0.9 +0.1 N.D.
carbon source L-tyrosine 10 mM (C) -0.9 -0.5 -0.7 -0.2 N.D.
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.2 -0.8 -0.4 -1.1 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.6 -0.6 -0.5 -0.3 N.D.
stress Glucose (C) and L-Glutamine (N); with TAPS; with chloride -0.2 -0.7 -0.4 -0.5 N.D.
carbon source Xanthosine (C) +0.3 -0.1 -1.0 -0.9 N.D.
stress Betaine (C)(N); with MOPS; with chloride -0.7 -0.5 +0.1 -0.6 N.D.
carbon source p-Coumaric (C) 5 mM -0.9 -0.1 -0.9 +0.3 N.D.
carbon source 4-Guanidinobutyric (C) +0.7 -1.0 -0.4 -0.9 N.D.
carbon source L-tyrosine 10 mM (C) -0.3 -0.6 -0.8 +0.1 N.D.
stress Betaine (C)(N); with TAPS; with chloride +0.3 -0.7 -0.7 -0.4 N.D.
carbon source L-Lysine (C) -0.1 +0.1 +0.2 -1.4 N.D.
carbon and nitrogen source NAG carbon and (N) +0.6 -0.8 -0.6 -0.2 N.D.
stress Betaine (C)(N); with MOPS; with chloride +0.6 +0.4 -0.7 -0.8 N.D.
carbon source Phloretic Acid 2 mM (C) -0.3 -0.8 -0.2 +0.9 N.D.
carbon and nitrogen source NAG carbon and (N) +0.6 -0.9 -0.1 +0.2 N.D.
no stress control L-Glutamine (C)(N); with MOPS -1.1 +0.7 -0.0 +0.4 N.D.
carbon source Choline chloride 10 mM (C) +0.4 +0.3 -1.0 +0.6 N.D.
carbon source L-Valine 10 mM (C) -0.7 -0.2 +1.0 +0.3 N.D.
pH Growth at pH8 and (C) Trisodium citrate -0.5 -0.3 +0.4 +0.9 N.D.
nitrogen source Ammonium chloride (N); with MOPS +0.2 -0.3 -0.4 +0.9 N.D.
nitrogen source NAG (N); with MOPS +0.5 +0.2 +0.5 -0.6 N.D.
carbon source p-Coumaric 2 mM (C) +0.8 +0.2 -0.5 +0.4 N.D.
carbon source 2-Deoxy-D-Ribose 10 mM (C) -0.4 +0.6 +0.2 +0.6 N.D.
temperature shift Temperature shift 10_to_25; with MOPS +1.0 -0.2 +0.2 +0.5 N.D.
carbon source L-Carnitine (C) +0.3 +0.4 -0.5 +1.3 N.D.
carbon source Xanthosine (C) -0.1 +0.4 +0.3 +0.9 N.D.
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