Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS15645 and PFLU_RS15650 are separated by 18 nucleotidesPFLU_RS15650 and PFLU_RS15655 are separated by 69 nucleotidesPFLU_RS15655 and PFLU_RS15660 are separated by 11 nucleotidesPFLU_RS15660 and PFLU_RS15665 are separated by 34 nucleotides PFLU_RS15645: PFLU_RS15645 - GTP-binding protein, at 3,514,751 to 3,516,007 _RS15645 PFLU_RS15650: PFLU_RS15650 - PAS domain-containing protein, at 3,516,026 to 3,517,318 _RS15650 PFLU_RS15655: PFLU_RS15655 - AMP-binding protein, at 3,517,388 to 3,519,028 _RS15655 PFLU_RS15660: PFLU_RS15660 - helix-turn-helix transcriptional regulator, at 3,519,040 to 3,519,615 _RS15660 PFLU_RS15665: PFLU_RS15665 - multidrug efflux RND transporter permease subunit, at 3,519,650 to 3,522,760 _RS15665
Group Condition PFLU_RS15645 PFLU_RS15650 PFLU_RS15655 PFLU_RS15660 PFLU_RS15665
no stress control Glycine betaine (C)(N); with TAPS -0.9 -0.2 -0.4 -1.0 +0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.6 -0.0 -0.0 -1.3 +0.0
carbon source Shikimic Acid (C) -0.4 +0.4 -0.7 -0.3 -0.7
stress Betaine (C)(N); with TAPS; with chloride -0.5 -0.1 -0.1 -1.0 -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.4 -0.5 -0.5 -0.4 +0.1
temperature Growth at 25C +0.0 -0.4 +0.2 -1.2 -0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.4 -0.3 -0.4 -1.4 +0.0
nitrogen source nitrate (N); with MOPS -0.3 -0.6 +0.3 -0.9 -0.2
temperature Growth at 25C; with MOPS -0.5 +0.0 -0.1 -1.0 -0.0
carbon source L-Isoleucine (C) -0.0 +0.3 -0.4 -1.0 -0.3
no stress control Glycine betaine (C)(N); with MOPS +0.2 -0.1 +0.1 -1.4 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.0 +0.4 -0.3 -0.5 +0.2
pH Growth at pH9 and (C) Trisodium citrate +0.2 -0.6 +0.2 -0.7 -0.2
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.1 +0.3 -0.6 -0.8 +0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.3 +0.2 -0.0 -1.6 +0.1
carbon source L-Carnitine (C) -0.8 +0.4 +0.2 -0.8 -0.0
stress Betaine (C)(N); with MOPS; with chloride +0.1 +0.6 -0.2 -1.1 -0.2
carbon source D,L-Malic Acid (C) -0.2 -0.3 +0.3 -0.7 +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.0 +0.2 +0.2 -0.5 +0.3
stress Betaine (C)(N); with TAPS; with chloride -0.6 -0.6 -0.2 +0.4 +0.3
carbon source Phloretic Acid 2 mM (C) +0.4 +0.2 +0.1 -1.2 -0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.3 -0.6 -0.4 +0.3 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.3 +0.4 +0.1 -0.8 +0.4
carbon source Cytidine 10 mM (C) -0.8 -0.4 +0.0 +0.7 +0.2
pH Growth at pH9 and (C) Trisodium citrate -1.0 +0.4 +0.1 +0.1 +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.0 +0.2 +0.8 -0.7 +0.1
carbon source Ferulic Acid (C) 1.5 mM +0.4 -0.4 -0.1 +0.8 +0.1
pH Growth at pH8 and (C) Trisodium citrate +1.0 -0.4 +0.3 +0.3 -0.0
carbon source 2-Deoxy-D-Ribose 10 mM (C) +0.5 -0.2 +0.1 +1.0 -0.2
carbon source Xanthosine (C) +1.0 +0.2 +0.7 +0.2 +0.1
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