Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS15310 and PFLU_RS15315 are separated by 128 nucleotidesPFLU_RS15315 and PFLU_RS15320 are separated by 36 nucleotidesPFLU_RS15320 and PFLU_RS15325 are separated by 2 nucleotidesPFLU_RS15325 and PFLU_RS15330 overlap by 4 nucleotides PFLU_RS15310: PFLU_RS15310 - YgdI/YgdR family lipoprotein, at 3,431,347 to 3,431,571 _RS15310 PFLU_RS15315: PFLU_RS15315 - lipase, at 3,431,700 to 3,433,109 _RS15315 PFLU_RS15320: PFLU_RS15320 - TolC family outer membrane protein, at 3,433,146 to 3,434,474 _RS15320 PFLU_RS15325: PFLU_RS15325 - HlyD family type I secretion periplasmic adaptor subunit, at 3,434,477 to 3,435,790 _RS15325 PFLU_RS15330: PFLU_RS15330 - type I secretion system permease/ATPase, at 3,435,787 to 3,437,538 _RS15330
Group Condition PFLU_RS15310 PFLU_RS15315 PFLU_RS15320 PFLU_RS15325 PFLU_RS15330
temperature Growth at 25C -3.3 -0.0 -0.2 -0.7 -0.6
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS -3.7 -0.1 +0.2 +0.0 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -3.0 -0.5 -0.1 -0.4 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -3.0 -0.1 +0.2 -0.6 -0.3
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -3.7 +0.0 +0.0 +0.3 -0.1
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS -2.9 +0.1 -0.1 -0.2 -0.3
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -2.1 -0.3 -0.1 -0.3 -0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.6 -0.7 -0.3 -0.3 -1.0
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -2.6 -0.2 -0.1 +0.0 -0.1
carbon source D,L-Malic Acid (C) -2.7 -0.5 +0.0 +0.0 +0.3
stress L-Glutamine (C)(N); with MOPS; with chloride -2.9 +0.2 +0.1 +0.1 -0.3
carbon source Quinic Acid (C) -3.1 +0.2 -0.1 +0.1 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -2.4 +0.2 -0.1 +0.1 -0.4
carbon source p-Coumaric 2 mM (C) -2.5 -0.2 -0.1 +0.6 -0.3
carbon source 2-Keto-D-gluconic hemicalcium salt (C) -2.5 +0.1 -0.0 +0.5 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -2.6 +0.0 +0.0 +0.4 -0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -2.0 +0.3 -0.3 -0.1 -0.2
stress Glucose (C) and Glycine betaine (N); with TAPS; with chloride -2.4 +0.0 +0.2 +0.1 -0.2
stress L-Glutamine (C)(N); with TAPS; with chloride -2.0 +0.3 +0.0 +0.2 -0.6
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -2.7 +0.3 -0.1 +0.5 -0.1
nitrogen source NAG (N); with MOPS -1.9 +0.5 -0.3 +0.1 -0.1
carbon source propionate 20 mM (C) -2.2 +0.9 -0.1 -0.0 -0.2
carbon source L-Isoleucine (C) -1.9 +0.4 -0.2 +0.7 -0.5
carbon source L-Carnitine (C) -2.1 +0.2 +0.1 -0.3 +0.6
carbon source Shikimic Acid (C) -2.2 -0.0 +0.3 +0.3 +0.3
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -2.1 +0.1 +0.1 +0.4 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +1.4 +0.4 -0.1 +0.2 -0.9
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +1.7 -0.6 +0.4 +0.5 -0.2
carbon source D-Glucosamine Hydrochloride 10 mM (C) +1.9 +0.2 -0.1 +0.4 -0.6
carbon source Inosine (C) +1.6 +0.5 +0.1 -0.4 +1.0
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