Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS14555 and PFLU_RS14560 are separated by 0 nucleotidesPFLU_RS14560 and PFLU_RS14565 are separated by 170 nucleotidesPFLU_RS14565 and PFLU_RS14570 are separated by 144 nucleotidesPFLU_RS14570 and PFLU_RS14575 are separated by 127 nucleotides PFLU_RS14555: PFLU_RS14555 - glutathione-disulfide reductase, at 3,253,466 to 3,254,824 _RS14555 PFLU_RS14560: PFLU_RS14560 - DNA-binding protein, at 3,254,825 to 3,255,832 _RS14560 PFLU_RS14565: PFLU_RS14565 - site-specific integrase, at 3,256,003 to 3,256,935 _RS14565 PFLU_RS14570: PFLU_RS14570 - peroxiredoxin, at 3,257,080 to 3,257,643 _RS14570 PFLU_RS14575: PFLU_RS14575 - alkyl hydroperoxide reductase subunit F, at 3,257,771 to 3,259,336 _RS14575
Group Condition PFLU_RS14555 PFLU_RS14560 PFLU_RS14565 PFLU_RS14570 PFLU_RS14575
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.9 -1.2 +0.3 -1.8 -0.6
stress Betaine (C)(N); with MOPS; with chloride -1.1 -1.6 +0.4 -0.5 -1.2
stress Betaine (C)(N); with MOPS; with chloride -1.2 -1.5 +0.1 -1.2 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.3 -0.3 -0.1 -1.0 -1.1
carbon source Xanthosine (C) -0.5 -1.4 -0.1 -0.4 -1.3
no stress control Glycine betaine (C)(N); with MOPS -1.8 -0.7 +0.1 -0.6 -0.4
stress Betaine (C)(N); with TAPS; with chloride -1.3 -0.6 -0.0 -0.3 -0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.1 -0.7 -0.3 -0.8 -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -1.0 -0.9 +0.2 -1.2 +0.0
carbon source acetate (C) -0.8 -1.5 -0.0 -0.5 -0.0
carbon source L-Valine (C) -0.9 -1.7 +0.0 +0.2 -0.4
stress Betaine (C)(N); with TAPS; with chloride -0.7 -1.6 +0.1 -0.6 +0.2
stress Betaine (C)(N); with TAPS; with chloride -1.3 -1.1 -0.0 -0.3 +0.3
carbon source L-Carnitine (C) -0.2 -2.0 +0.2 -0.7 +0.2
pH Growth at pH8 and (C) Trisodium citrate -0.4 -1.8 -0.1 +0.5 -0.5
stress L-Glutamine (C)(N); with TAPS; with chloride -1.3 -0.7 +0.3 -0.4 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.7 +0.5 +0.0 -1.1 -0.8
stress Betaine (C)(N); with MOPS; with chloride -1.1 -1.1 -0.4 +0.0 +0.6
stress Betaine (C)(N); with TAPS; with chloride -0.6 -1.0 -0.2 -0.5 +0.5
carbon source Xanthosine (C) -0.6 -1.6 +0.4 +0.6 -0.6
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -1.8 +0.7 -0.0 -0.4 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.7 +0.7 -0.3 -1.2 -0.1
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -1.4 -0.7 +0.5 +0.4 -0.0
carbon source Shikimic Acid (C) -0.1 -1.5 -0.4 +0.9 -0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -1.1 -0.7 +0.4 +0.4 -0.1
no stress control Glycine betaine (C)(N); with TAPS +0.6 +0.1 +0.4 -1.9 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.0 -0.6 +0.7 +0.7 -0.3
stress Betaine (C)(N); with TAPS; with chloride -1.2 -0.4 +0.2 +0.7 +0.3
carbon source propionate 20 mM (C) +1.5 -0.5 +0.2 -0.4 -0.2
carbon source propionate 20 mM (C) +1.5 +0.6 -0.3 -0.5 +0.5
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