Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS14185 and PFLU_RS14190 are separated by 3 nucleotidesPFLU_RS14190 and PFLU_RS14195 are separated by 183 nucleotidesPFLU_RS14195 and PFLU_RS14200 are separated by 12 nucleotidesPFLU_RS14200 and PFLU_RS14205 are separated by 68 nucleotides PFLU_RS14185: PFLU_RS14185 - DNA ligase D, at 3,172,262 to 3,174,778 _RS14185 PFLU_RS14190: PFLU_RS14190 - Ku protein, at 3,174,782 to 3,175,615 _RS14190 PFLU_RS14195: PFLU_RS14195 - hypothetical protein, at 3,175,799 to 3,176,113 _RS14195 PFLU_RS14200: PFLU_RS14200 - PQQ-dependent sugar dehydrogenase, at 3,176,126 to 3,177,283 _RS14200 PFLU_RS14205: PFLU_RS14205 - nuclear transport factor 2 family protein, at 3,177,352 to 3,177,831 _RS14205
Group Condition PFLU_RS14185 PFLU_RS14190 PFLU_RS14195 PFLU_RS14200 PFLU_RS14205
stress Glucose (C) and Betaine (N); with TAPS; with chloride -0.0 -0.5 -4.3 -0.2 -0.5
carbon source propionate 20 mM (C) -0.1 -0.4 -3.9 -0.0 -0.0
no stress control L-Glutamine (C)(N); with MOPS +0.2 -0.0 -4.7 +0.2 -0.1
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.2 -0.0 -4.5 +0.3 +0.1
carbon source Ferulic Acid 2 mM (C) -0.1 +0.2 -3.7 -0.1 -0.5
carbon source Xanthosine (C) +0.1 -0.4 -2.9 -0.6 -0.1
pH Growth at pH9 and (C) Trisodium citrate -0.3 -0.5 -2.9 -0.0 -0.1
carbon source NAG (C) -0.1 -0.4 -3.0 -0.0 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.0 +0.1 -1.9 -0.5 -1.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.4 -0.6 -3.1 -0.3 +0.0
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS -0.0 -0.3 -3.1 -0.1 +0.0
no stress control Glycine betaine (C)(N); with TAPS -0.1 +0.1 -1.5 -1.1 -0.6
temperature Growth at 30C -0.1 +0.1 -3.0 +0.3 -0.4
carbon source D-(+)-Galactosamine 10 mM (C) +0.1 +0.4 -2.9 -0.3 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.2 +0.5 -2.3 -0.5 -0.8
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.1 -0.2 -2.6 -0.1 +0.2
carbon source 2-Keto-D-gluconic hemicalcium salt (C) +0.2 +0.3 -3.1 +0.1 -0.2
carbon source D-Xylose (C) -0.0 +0.3 -2.7 -0.2 -0.1
carbon source L-Isoleucine (C) +0.1 +0.4 -1.6 -0.4 -1.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.0 +0.6 -2.5 +0.1 -0.6
carbon source D,L-Malic Acid (C) -0.3 +0.4 -1.4 -0.3 -0.9
carbon source Phloretic Acid 2 mM (C) +0.1 +0.4 -2.4 -0.3 -0.3
carbon source caffeic 2 mM (C) -0.1 +0.3 -2.1 +0.5 -0.9
no stress control L-Glutamine (C)(N); with TAPS +0.1 +0.5 -1.7 -0.0 -1.0
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS +0.1 +0.9 -2.7 +0.6 -0.9
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.2 +0.6 -0.9 +0.6 -1.6
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.3 +1.0 -2.0 +0.0 +0.3
carbon source 4-Aminobutyric (C) +0.5 +1.0 -1.6 +0.1 -0.7
stress Betaine (C)(N); with MOPS; with chloride +0.3 +1.0 -2.3 +0.8 -0.3
carbon source L-Valine (C) -0.1 -0.1 +2.9 -0.2 -0.4
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