Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS13630 and PFLU_RS13635 are separated by 357 nucleotidesPFLU_RS13635 and PFLU_RS13640 are separated by 177 nucleotidesPFLU_RS13640 and PFLU_RS13645 are separated by 183 nucleotidesPFLU_RS13645 and PFLU_RS13650 are separated by 14 nucleotides PFLU_RS13630: PFLU_RS13630 - putative C-S lyase, at 3,081,943 to 3,083,088 _RS13630 PFLU_RS13635: PFLU_RS13635 - MFS transporter, at 3,083,446 to 3,084,756 _RS13635 PFLU_RS13640: PFLU_RS13640 - isochorismatase family protein, at 3,084,934 to 3,085,566 _RS13640 PFLU_RS13645: PFLU_RS13645 - Asp/Glu racemase, at 3,085,750 to 3,086,502 _RS13645 PFLU_RS13650: PFLU_RS13650 - alpha/beta hydrolase, at 3,086,517 to 3,087,314 _RS13650
Group Condition PFLU_RS13630 PFLU_RS13635 PFLU_RS13640 PFLU_RS13645 PFLU_RS13650
carbon source Inosine (C) -0.3 -0.1 -3.4 +0.5 -1.1
nitrogen source NAG (N); with MOPS -0.6 -0.0 -3.2 -0.2 -0.2
stress L-Glutamine (C)(N); with TAPS; with chloride -0.0 -0.3 -3.4 +0.1 -0.2
carbon source Inosine (C) -0.2 +0.1 -4.1 +0.3 +0.3
carbon source propionate 20 mM (C) -0.2 +0.1 -3.0 -0.3 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.4 -0.3 -2.5 +0.0 +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.4 -0.3 -0.8 -0.9 -0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.2 +0.2 -2.2 -0.6 -0.0
carbon and nitrogen source NAG carbon and (N); with MOPS +0.1 -0.1 -2.2 -0.0 -0.5
carbon and nitrogen source NAG carbon and (N); with MOPS -0.3 -0.3 -1.9 -0.2 -0.1
pH Growth at pH6 and (C) D-Glucose -0.2 -0.5 -2.4 +0.4 +0.0
stress L-Glutamine (C)(N); with MOPS; with chloride -0.3 +0.1 -1.9 -0.4 +0.0
temperature Growth at 25C -0.1 +0.0 -2.5 +0.2 +0.0
carbon source L-Carnitine (C) +0.3 +0.0 -2.4 +0.2 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.0 -0.3 -2.4 +0.2 +0.4
carbon source 4-Aminobutyric (C) +0.3 -0.6 -1.3 +0.1 -0.7
temperature shift Temperature shift 10_to_25; with MOPS +0.3 -0.1 -1.5 -0.4 -0.3
stress Betaine (C)(N); with MOPS; with chloride +0.4 +0.2 -1.3 -0.7 -0.2
pH Growth at pH9 and (C) D-Glucose -0.0 +0.2 -1.8 +0.4 -0.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.2 +0.2 -2.4 +0.5 +0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.5 -0.2 -1.7 +0.5 +0.7
stress Betaine (C)(N); with TAPS; with chloride +0.4 +0.5 -1.7 +0.1 +0.6
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.5 -0.4 +1.2 -0.4 +0.1
carbon source L-Valine (C) +0.6 +0.4 -1.0 +0.9 +0.0
carbon source Phloretic Acid 2 mM (C) +0.2 +0.2 +1.8 -0.7 +0.4
stress Betaine (C)(N); with MOPS; with chloride +0.1 -0.1 +1.8 -0.1 +0.5
carbon source p-Coumaric (C) 5 mM +0.5 +0.3 +1.7 +0.1 -0.4
carbon source L-Valine (C) +0.4 -0.5 +0.7 +1.4 +0.5
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.3 -0.2 +1.4 +0.5 +0.5
carbon source L-Valine 10 mM (C) +0.3 +0.3 +1.9 +1.1 +0.2
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