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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens SBW25-INTG
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PFLU_RS13300 and PFLU_RS13305 overlap by 4 nucleotides
PFLU_RS13305 and PFLU_RS13310 are separated by 50 nucleotides
PFLU_RS13310 and PFLU_RS13315 are separated by 138 nucleotides
PFLU_RS13315 and PFLU_RS13320 are separated by 91 nucleotides
PFLU_RS13300: PFLU_RS13300 - DNA-directed RNA polymerase sigma-70 factor, at 3,008,411 to 3,009,079
_RS13300
PFLU_RS13305: PFLU_RS13305 - sigma-70 family RNA polymerase sigma factor, at 3,009,076 to 3,009,591
_RS13305
PFLU_RS13310: PFLU_RS13310 - DUF3455 domain-containing protein, at 3,009,642 to 3,010,163
_RS13310
PFLU_RS13315: PFLU_RS13315 - class I SAM-dependent methyltransferase, at 3,010,302 to 3,011,072
_RS13315
PFLU_RS13320: PFLU_RS13320 - sulfite exporter TauE/SafE family protein, at 3,011,164 to 3,011,949
_RS13320
Group
Condition
PFLU
_RS13300
PFLU
_RS13305
PFLU
_RS13310
PFLU
_RS13315
PFLU
_RS13320
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
-0.8
-0.2
-1.1
-1.5
-0.1
pH
Growth at pH9 and (C) Trisodium citrate
-3.5
-0.4
+0.2
+0.5
-0.1
stress
Betaine (C)(N); with TAPS; with chloride
-2.2
-0.9
+0.3
+0.2
-0.7
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
+0.2
-2.0
-0.1
-1.7
+0.4
carbon source
L-Valine (C)
-1.6
-0.5
-0.1
-0.8
-0.1
stress
L-Glutamine (C)(N); with MOPS; with chloride
-2.0
-1.0
+0.2
-0.2
-0.1
pH
Growth at pH8 and (C) Trisodium citrate
-2.9
+0.2
+0.6
-1.4
+0.5
carbon source
2-Deoxy-D-Ribose 10 mM (C)
+0.6
-0.5
-0.5
-2.2
-0.3
carbon source
NAG (C)
-2.6
+0.1
-0.3
+0.2
-0.1
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
-2.1
-0.7
+0.0
+0.6
-0.2
stress
Glucose (C) and Betaine (N); with TAPS; with chloride
-2.4
+0.4
+0.1
+0.0
-0.5
no stress control
D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS
-1.8
-0.7
+0.1
+0.4
-0.2
pH
Growth at pH9 and (C) Trisodium citrate
-2.4
-0.4
-0.5
+0.9
+0.4
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
-1.4
-1.3
+0.3
-0.0
+0.5
carbon source
4-Hydroxybenzoic Acid 2 mM (C)
-2.3
+0.6
-0.3
+0.3
+0.2
carbon source
Xanthosine (C)
-1.1
-1.2
+0.8
-0.2
+0.4
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
-1.6
-0.4
+0.5
+0.8
-0.6
carbon source
Xanthosine (C)
+0.7
-1.4
-0.7
+0.2
+0.6
no stress control
Glycine betaine (C)(N); with MOPS
-0.8
+0.4
+0.6
+0.8
-1.0
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride
+1.4
+0.3
+0.1
-1.9
+0.5
stress
Betaine (C)(N); with MOPS; with chloride
-1.3
+0.5
+0.4
+0.3
+0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.8
+1.1
-0.4
+0.7
+0.1
no stress control
Glycine betaine (C)(N); with MOPS
-1.5
+0.7
-0.1
+1.3
+0.4
carbon source
p-Coumaric (C) 5 mM
+0.8
-1.1
-0.2
+1.1
+0.1
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
+1.0
+0.8
+0.3
-0.9
-0.2
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
+0.7
-0.8
+0.3
+1.0
+0.4
carbon source
Choline chloride 10 mM (C)
+0.9
+0.5
+0.3
+1.1
-0.7
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
+0.9
+0.7
-0.6
+0.7
+0.6
stress
Betaine (C)(N); with MOPS; with chloride
+1.8
-0.2
+0.0
+0.6
+0.5
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
+0.4
+0.8
+0.4
+1.0
+0.6
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