Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS13115 and PFLU_RS13120 are separated by 106 nucleotidesPFLU_RS13120 and PFLU_RS13125 are separated by 145 nucleotidesPFLU_RS13125 and PFLU_RS13130 are separated by 114 nucleotidesPFLU_RS13130 and PFLU_RS31590 are separated by 160 nucleotides PFLU_RS13115: PFLU_RS13115 - GNAT family N-acetyltransferase, at 2,963,464 to 2,963,955 _RS13115 PFLU_RS13120: PFLU_RS13120 - hypothetical protein, at 2,964,062 to 2,964,283 _RS13120 PFLU_RS13125: PFLU_RS13125 - TonB-dependent siderophore receptor, at 2,964,429 to 2,966,672 _RS13125 PFLU_RS13130: PFLU_RS13130 - phosphoethanolamine transferase CptA, at 2,966,787 to 2,968,535 _RS13130 PFLU_RS31590: PFLU_RS31590 - hypothetical protein, at 2,968,696 to 2,968,875 _RS31590
Group Condition PFLU_RS13115 PFLU_RS13120 PFLU_RS13125 PFLU_RS13130 PFLU_RS31590
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. -5.1 -0.0 -0.1 -3.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. -3.0 +0.1 -0.0 -3.7
carbon source NAG (C) N.D. -2.6 -0.0 -0.2 -3.3
stress L-Glutamine (C)(N); with MOPS; with chloride N.D. -3.9 +0.1 -0.3 -2.0
no stress control L-Glutamine (C)(N); with TAPS N.D. -3.2 -0.1 -0.1 -2.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. -4.2 +0.0 +0.0 -1.2
stress Betaine (C)(N); with TAPS; with chloride N.D. -2.3 +0.0 -0.0 -3.0
carbon source Inosine (C) N.D. -3.2 +0.1 +0.1 -2.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. -3.2 +0.0 +0.0 -1.9
carbon source acetate (C) N.D. -1.6 -0.0 +0.0 -3.5
carbon source D-Xylose (C) N.D. -4.1 -0.1 +0.1 -0.9
no stress control Sucrose (C) and Ammonium chloride (N); with TAPS N.D. -5.3 -0.0 -0.1 +0.4
carbon source 2-Keto-D-gluconic hemicalcium salt (C) N.D. -1.9 +0.0 -0.1 -3.0
pH Growth at pH9 and (C) Trisodium citrate N.D. -2.8 -0.1 -0.0 -1.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs N.D. -1.0 +0.0 -0.0 -3.5
carbon source D-(+)-Galactosamine (C) N.D. -2.7 -0.1 -0.2 -1.6
carbon source 4-Guanidinobutyric (C) N.D. -3.7 -0.1 +0.0 -0.8
carbon and nitrogen source NAG carbon and (N); with MOPS N.D. -2.8 +0.0 +0.0 -1.4
carbon source m-Inositol (C) N.D. -0.7 +0.2 -0.2 -3.3
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS N.D. +0.4 -0.2 +0.1 -3.9
carbon source L-Malic 20 mM (C) N.D. +0.7 +0.0 -0.1 -4.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -3.8 +0.0 -0.1 +0.8
temperature shift Temperature shift 10_to_25; with MOPS N.D. +0.7 -0.1 -0.0 -3.6
stress Sucrose (C) and L-Glutamine (N); with MOPS; with PEG N.D. +0.7 -0.0 +0.0 -3.6
pH Growth at pH6 and (C) D-Glucose N.D. -3.5 -0.0 +0.2 +1.0
carbon source Phloretic Acid 2 mM (C) N.D. +1.2 -0.3 -0.1 -3.0
carbon source acetate (C) N.D. +1.4 +0.0 -0.0 -3.2
nitrogen source NAG (N); with MOPS N.D. +1.7 -0.3 +0.2 -2.9
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride N.D. +1.8 +0.0 +0.1 -3.2
carbon source p-Coumaric (C) 5 mM N.D. -2.5 +0.2 +0.5 +1.9
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