Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS12770 and PFLU_RS30765 are separated by 192 nucleotidesPFLU_RS30765 and PFLU_RS12775 are separated by 288 nucleotidesPFLU_RS12775 and PFLU_RS30770 overlap by 14 nucleotidesPFLU_RS30770 and PFLU_RS12780 are separated by 73 nucleotides PFLU_RS12770: PFLU_RS12770 - protease SohB, at 2,880,698 to 2,881,723 _RS12770 PFLU_RS30765: PFLU_RS30765 - DAK2 domain-containing protein, at 2,881,916 to 2,882,026 _RS30765 PFLU_RS12775: PFLU_RS12775 - TerC family protein, at 2,882,315 to 2,883,883 _RS12775 PFLU_RS30770: PFLU_RS30770 - thioredoxin family protein, at 2,883,870 to 2,884,196 _RS30770 PFLU_RS12780: PFLU_RS12780 - hypothetical protein, at 2,884,270 to 2,884,518 _RS12780
Group Condition PFLU_RS12770 PFLU_RS30765 PFLU_RS12775 PFLU_RS30770 PFLU_RS12780
stress Betaine (C)(N); with MOPS; with chloride -1.0 N.D. -0.0 -2.4 -0.6
carbon source Phloretic Acid 2 mM (C) -0.5 N.D. -0.4 -2.4 -0.3
carbon source L-Carnitine (C) -0.5 N.D. -0.2 -2.4 -0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.0 N.D. +0.1 -1.8 -0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.2 N.D. -0.3 -2.3 -0.1
stress Betaine (C)(N); with TAPS; with chloride -0.6 N.D. -0.4 -0.9 -0.2
carbon source L-Carnitine (C) -0.6 N.D. +0.6 -1.5 -0.6
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.0 N.D. +0.3 -1.9 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.4 N.D. -0.7 -1.1 +0.9
carbon source D-(+)-Galactosamine 10 mM (C) +0.4 N.D. +0.2 -1.3 -0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.6 N.D. +0.3 -1.2 -0.3
carbon source p-Coumaric 2 mM (C) -0.1 N.D. -0.6 +0.9 -0.8
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.3 N.D. -0.0 -1.0 +0.9
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.6 N.D. -0.9 +0.6 +0.5
carbon source Cytidine 10 mM (C) -0.7 N.D. +0.4 +0.8 -0.5
carbon source pyruvate 20 mM (C) +0.1 N.D. -1.1 +0.8 +0.3
carbon source 4-Guanidinobutyric (C) -1.0 N.D. +0.3 +1.1 -0.3
carbon source Xanthosine (C) -0.2 N.D. +0.7 +0.9 -0.7
stress Betaine (C)(N); with TAPS; with chloride -0.0 N.D. +0.6 +1.0 -0.7
carbon source Xanthosine (C) -0.6 N.D. +0.6 +1.1 -0.1
carbon source NAG (C) -0.5 N.D. +0.4 +0.5 +0.7
carbon and nitrogen source NAG carbon and (N) -0.4 N.D. -0.4 +1.5 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 N.D. +1.2 +0.5 -0.3
stress Betaine (C)(N); with MOPS; with chloride -0.6 N.D. +0.4 +1.5 +0.1
temperature Growth at 25C +0.8 N.D. -0.5 +0.9 +0.3
stress Betaine (C)(N); with MOPS; with chloride -0.4 N.D. +0.6 +1.0 +0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.5 N.D. -0.0 +1.8 +0.4
pH Growth at pH9 and (C) Trisodium citrate +0.5 N.D. -0.8 +1.8 +0.4
stress Betaine (C)(N); with MOPS; with chloride +0.3 N.D. +0.7 +1.3 +0.2
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.6 N.D. +1.0 +0.8 +0.3
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