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Fitness for 5 genes in
Pseudomonas fluorescens SBW25-INTG
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PFLU_RS12705 and PFLU_RS12710 are separated by 109 nucleotides
PFLU_RS12710 and PFLU_RS12715 are separated by 66 nucleotides
PFLU_RS12715 and PFLU_RS12720 are separated by 36 nucleotides
PFLU_RS12720 and PFLU_RS12725 are separated by 77 nucleotides
PFLU_RS12705: PFLU_RS12705 - LysR family transcriptional regulator, at 2,871,374 to 2,872,273
_RS12705
PFLU_RS12710: PFLU_RS12710 - AbrB family transcriptional regulator, at 2,872,383 to 2,873,435
_RS12710
PFLU_RS12715: PFLU_RS12715 - fumarylacetoacetate hydrolase family protein, at 2,873,502 to 2,874,197
_RS12715
PFLU_RS12720: PFLU_RS12720 - AraC family transcriptional regulator, at 2,874,234 to 2,875,142
_RS12720
PFLU_RS12725: PFLU_RS12725 - GNAT family N-acetyltransferase, at 2,875,220 to 2,875,429
_RS12725
Group
Condition
PFLU
_RS12705
PFLU
_RS12710
PFLU
_RS12715
PFLU
_RS12720
PFLU
_RS12725
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.3
-1.0
-3.9
+0.2
-0.6
stress
Betaine (C)(N); with TAPS; with chloride
+0.1
-1.6
-4.1
+0.0
+0.2
stress
Glucose (C) and L-Glutamine (N); with MOPS; with chloride
+0.3
-0.2
-3.2
-0.1
-1.7
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
-0.3
-1.2
-2.3
-0.2
-0.8
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
-0.9
+0.8
-3.7
+0.4
-1.3
temperature shift
Temperature shift 10_to_25; with MOPS
-0.2
-0.0
-3.0
-0.2
-1.1
temperature
Growth at 25C
-0.1
-0.6
-3.0
-0.1
-0.5
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
-0.6
-1.3
-3.3
+0.2
+0.6
no stress control
L-Glutamine (C)(N); with MOPS
+0.5
-0.0
-3.3
-0.1
-1.1
carbon source
caffeic 2 mM (C)
+0.1
+0.7
-3.2
+0.0
-1.4
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
+0.3
+0.2
-2.8
+0.1
-1.2
no stress control
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS
+0.6
-3.7
+0.1
-0.1
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.3
+0.4
-3.7
-0.1
+0.1
nitrogen source
Ammonium chloride (N); with MOPS
+0.5
-0.4
-2.9
-0.2
+0.3
stress
L-Glutamine (C)(N); with MOPS; with chloride
-0.1
-0.0
-3.6
-0.0
+1.0
carbon source
p-Coumaric 2 mM (C)
+0.2
+0.5
-2.0
+0.4
-1.6
carbon source
Xanthosine (C)
+0.1
+1.2
-3.5
-0.4
+0.1
stress
Betaine (C)(N); with MOPS; with chloride
+0.1
+1.2
-0.9
-0.2
-2.4
stress
L-Glutamine (C)(N); with TAPS; with chloride
-0.2
-0.6
-2.6
-0.4
+1.7
stress
Betaine (C)(N); with MOPS; with chloride
+0.5
+1.0
-3.2
+0.1
-0.5
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride
-0.4
+1.2
-3.6
+0.6
+0.1
carbon source
Phloretic Acid 2 mM (C)
+0.5
+1.1
-1.7
-0.2
-1.1
pH
Growth at pH9 and (C) Trisodium citrate
+0.0
+1.1
+1.0
-0.1
-3.5
carbon source
4-Guanidinobutyric (C)
+0.4
-0.5
-2.6
+0.8
+0.6
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
+0.2
-0.5
-2.2
+0.1
+1.1
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-0.1
-0.3
+1.4
+0.1
-2.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.3
-1.0
+1.7
-0.1
-1.2
carbon source
Shikimic Acid (C)
+0.3
-2.2
+1.8
+0.5
-0.2
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
+0.7
-0.3
-1.8
+0.7
+1.1
carbon source
Phloretic Acid 2 mM (C)
+0.5
+0.3
+1.5
+0.4
-1.6
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