Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS12700 and PFLU_RS12705 are separated by 4 nucleotidesPFLU_RS12705 and PFLU_RS12710 are separated by 109 nucleotidesPFLU_RS12710 and PFLU_RS12715 are separated by 66 nucleotidesPFLU_RS12715 and PFLU_RS12720 are separated by 36 nucleotides PFLU_RS12700: PFLU_RS12700 - hypothetical protein, at 2,871,040 to 2,871,369 _RS12700 PFLU_RS12705: PFLU_RS12705 - LysR family transcriptional regulator, at 2,871,374 to 2,872,273 _RS12705 PFLU_RS12710: PFLU_RS12710 - AbrB family transcriptional regulator, at 2,872,383 to 2,873,435 _RS12710 PFLU_RS12715: PFLU_RS12715 - fumarylacetoacetate hydrolase family protein, at 2,873,502 to 2,874,197 _RS12715 PFLU_RS12720: PFLU_RS12720 - AraC family transcriptional regulator, at 2,874,234 to 2,875,142 _RS12720
Group Condition PFLU_RS12700 PFLU_RS12705 PFLU_RS12710 PFLU_RS12715 PFLU_RS12720
stress Betaine (C)(N); with TAPS; with chloride +0.1 +0.1 -1.6 -4.1 +0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.4 -0.6 -1.3 -3.3 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.0 -0.3 -1.0 -3.9 +0.2
temperature Growth at 25C -0.2 -0.1 -0.6 -3.0 -0.1
stress L-Glutamine (C)(N); with TAPS; with chloride -0.2 -0.2 -0.6 -2.6 -0.4
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.2 -0.3 -1.2 -2.3 -0.2
stress Glucose (C) and L-Glutamine (N); with MOPS; with chloride -0.4 +0.3 -0.2 -3.2 -0.1
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS -0.3 -0.6 -0.8 -1.9 -0.0
stress L-Glutamine (C)(N); with MOPS; with chloride +0.2 -0.1 -0.0 -3.6 -0.0
pH Growth at pH7 and (C) D-Glucose -0.5 -0.1 -0.2 -2.8 +0.1
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS -0.3 +0.6 -3.7 +0.1 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.0 -0.9 +0.8 -3.7 +0.4
no stress control L-Glutamine (C)(N); with MOPS -0.5 +0.5 -0.0 -3.3 -0.1
carbon source Inosine 10 mM (C) +0.0 +0.2 -0.0 -3.4 -0.1
temperature shift Temperature shift 10_to_25; with MOPS +0.2 -0.2 -0.0 -3.0 -0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.1 -0.4 -0.0 -3.0 +0.3
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.1 +0.0 +0.3 -3.2 -0.0
nitrogen source Ammonium chloride (N); with MOPS +0.1 +0.5 -0.4 -2.9 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.2 +0.3 +0.4 -3.7 -0.1
stress Betaine (C)(N); with TAPS; with chloride +0.1 +0.2 -0.3 -3.0 +0.0
lb outgrowth control more dilute start LB outgrowth control more dilute start +0.2 +0.0 +0.3 -3.1 -0.1
carbon source 4-Guanidinobutyric (C) -0.5 +0.4 -0.5 -2.6 +0.8
carbon source caffeic 2 mM (C) +0.0 +0.1 +0.7 -3.2 +0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.1 -0.4 +1.2 -3.6 +0.6
carbon source Xanthosine (C) +0.3 +0.1 +1.2 -3.5 -0.4
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 +0.3 +0.2 -2.8 +0.1
stress Betaine (C)(N); with MOPS; with chloride -0.1 +0.5 +1.0 -3.2 +0.1
carbon source Phloretic Acid 2 mM (C) -0.3 +0.5 +1.1 -1.7 -0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.1 +0.7 -0.3 -1.8 +0.7
carbon source Shikimic Acid (C) +0.3 +0.3 -2.2 +1.8 +0.5
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