Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS12410 and PFLU_RS12415 overlap by 17 nucleotidesPFLU_RS12415 and PFLU_RS12420 are separated by 140 nucleotidesPFLU_RS12420 and PFLU_RS12425 are separated by 0 nucleotidesPFLU_RS12425 and PFLU_RS12430 overlap by 4 nucleotides PFLU_RS12410: PFLU_RS12410 - MATE family efflux transporter, at 2,751,363 to 2,752,718 _RS12410 PFLU_RS12415: PFLU_RS12415 - TetR/AcrR family transcriptional regulator, at 2,752,702 to 2,753,289 _RS12415 PFLU_RS12420: PFLU_RS12420 - MFS transporter, at 2,753,430 to 2,754,602 _RS12420 PFLU_RS12425: PFLU_RS12425 - type II toxin-antitoxin system HicB family antitoxin, at 2,754,603 to 2,754,968 _RS12425 PFLU_RS12430: PFLU_RS12430 - type II toxin-antitoxin system HicA family toxin, at 2,754,965 to 2,755,216 _RS12430
Group Condition PFLU_RS12410 PFLU_RS12415 PFLU_RS12420 PFLU_RS12425 PFLU_RS12430
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.1 -0.4 -0.8 -1.4 -1.3
stress Betaine (C)(N); with TAPS; with chloride -0.1 -0.6 -1.1 -0.1 -1.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 -1.1 -0.7 -0.8 -0.9
stress Betaine (C)(N); with MOPS; with chloride -0.2 -2.6 -0.3 -0.5 +0.5
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.7 -0.7 +0.1 -2.2 -0.6
carbon source propionate 20 mM (C) -0.0 -0.2 -0.5 -1.0 -0.9
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.4 -0.9 +0.4 -2.3 -0.2
stress Betaine (C)(N); with TAPS; with chloride +0.1 -1.2 +0.2 -0.2 -1.3
stress Betaine (C)(N); with MOPS; with chloride +0.2 -1.4 -0.0 -0.9 -0.0
stress Betaine (C)(N); with MOPS; with chloride +0.2 -2.2 +0.2 +0.3 -0.5
stress Betaine (C)(N); with TAPS; with chloride +0.1 -1.3 -0.2 -0.9 +0.2
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.8 -1.0 -0.1 -2.2 +0.4
stress Glucose (C) and Glycine betaine (N); with TAPS; with chloride +0.0 -2.0 -0.0 +0.1 -0.1
carbon source L-tyrosine 10 mM (C) +0.1 -1.1 +0.3 -0.8 -0.4
carbon source 4-Guanidinobutyric (C) +0.3 -0.2 +0.1 -0.5 -1.5
temperature Growth at 25C +0.2 -0.1 -0.4 -0.6 -1.0
no stress control Ferulic (C) and Ammonium chloride (N); with MOPS +0.1 -1.5 -0.1 -0.5 +0.3
stress Sucrose (C) and Glycine betaine (N); with TAPS -0.0 -1.9 +0.3 -0.1 +0.1
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.3 +0.2 -0.1 -0.4 -1.5
stress Glucose (C) and Betaine (N); with TAPS; with chloride +0.2 -0.7 -0.5 -0.7 +0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.2 -1.5 +0.5 -0.4 -0.2
carbon source Ferulic Acid (C) 1.5 mM +0.5 -1.4 +0.1 -0.3 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 -1.5 +0.4 +0.2 -0.4
carbon source Phloretic Acid 2 mM (C) +0.5 -0.6 -0.5 +0.3 -0.9
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.2 -1.0 -0.2 -0.3 +0.8
pH Growth at pH8 and (C) Trisodium citrate +0.7 +0.5 -0.5 -0.6 -0.8
stress Betaine (C)(N); with MOPS; with chloride +0.3 -0.7 -0.5 +0.9 -0.7
stress Betaine (C)(N); with MOPS; with chloride +0.2 -1.4 +0.3 +0.4 +0.2
carbon source L-Valine (C) -0.1 -0.1 +0.8 -1.1 +0.2
carbon source L-Carnitine (C) -0.4 -0.1 -0.8 +0.9 +0.5
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