Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS11980 and PFLU_RS11985 are separated by 11 nucleotidesPFLU_RS11985 and PFLU_RS11990 are separated by 64 nucleotidesPFLU_RS11990 and PFLU_RS11995 are separated by 72 nucleotidesPFLU_RS11995 and PFLU_RS12000 are separated by 222 nucleotides PFLU_RS11980: PFLU_RS11980 - precorrin-6A synthase (deacetylating), at 2,664,202 to 2,664,957 _RS11980 PFLU_RS11985: PFLU_RS11985 - phospholipase, at 2,664,969 to 2,665,688 _RS11985 PFLU_RS11990: PFLU_RS11990 - hypothetical protein, at 2,665,753 to 2,666,307 _RS11990 PFLU_RS11995: PFLU_RS11995 - potassium transporter, at 2,666,380 to 2,668,188 _RS11995 PFLU_RS12000: PFLU_RS12000 - hypothetical protein, at 2,668,411 to 2,668,776 _RS12000
Group Condition PFLU_RS11980 PFLU_RS11985 PFLU_RS11990 PFLU_RS11995 PFLU_RS12000
carbon source NAG (C) -2.2 -0.1 -0.3 -1.2 -1.3
carbon source Shikimic Acid (C) -0.8 -0.5 -1.0 -0.8 -1.7
pH Growth at pH9 and (C) Trisodium citrate -1.5 -0.2 -2.3 -0.0 -0.5
carbon and nitrogen source NAG carbon and (N) -2.5 -0.4 -0.6 +0.1 -1.0
carbon source p-Coumaric 2 mM (C) -1.4 -0.5 -0.9 -0.5 -0.9
carbon source L-Carnitine (C) -0.9 -0.1 -1.1 -0.5 -1.5
carbon source p-Coumaric (C) 1.5 mM -0.7 -0.1 -2.1 -0.4 -0.6
carbon source 4-hydroxyphenylacetic 2 mM (C) -1.4 -0.0 -1.0 -0.6 -0.7
carbon source propionate 20 mM (C) -1.0 -1.1 -0.7 -0.9 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 -0.7 -2.9 +0.4 -0.1
no stress control Glycine betaine (C)(N); with TAPS -0.7 -0.4 -0.7 -0.6 -1.1
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -0.9 -0.2 -1.6 -0.2 -0.6
carbon source acetate (C) -0.5 +0.5 -2.3 -1.0 -0.2
temperature Growth at 25C; with MOPS -0.4 -0.9 -1.8 +0.3 -0.7
stress Betaine (C)(N); with MOPS; with chloride -2.1 +0.4 -0.8 -0.2 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 -0.9 -2.5 +0.3 -0.5
carbon source NAG (C) -1.4 -1.0 -0.5 +0.2 -0.4
carbon and nitrogen source NAG carbon and (N) -1.7 -0.6 +0.3 -0.3 -0.7
temperature Growth at 30C -0.6 +0.1 -1.9 +0.2 -0.7
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -2.3 +0.2 +0.2 -0.5 -0.3
pH Growth at pH6 and (C) D-Glucose -1.9 +0.4 -1.1 +0.1 +0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -2.2 -0.5 +0.3 +0.6 -0.4
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -2.7 +0.4 -0.1 +0.3 +0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -2.4 -0.4 -0.1 +0.9 -0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.2 +0.5 -0.4 +0.3 -1.1
carbon source 4-Hydroxybenzoic Acid 2 mM (C) -2.0 +0.6 +0.4 -0.1 -0.7
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -1.8 +0.4 +0.8 +0.1 -1.2
stress Betaine (C)(N); with MOPS; with chloride -1.0 -0.6 -1.0 +0.3 +0.7
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -2.2 -0.4 +1.4 +0.6 -0.1
stress Betaine (C)(N); with MOPS; with chloride -1.5 +0.2 +1.6 +0.2 +0.3
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