Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS11705 and PFLU_RS11710 overlap by 4 nucleotidesPFLU_RS11710 and PFLU_RS11715 are separated by 186 nucleotidesPFLU_RS11715 and PFLU_RS11720 are separated by 1025 nucleotidesPFLU_RS11720 and PFLU_RS11725 are separated by 507 nucleotides PFLU_RS11705: PFLU_RS11705 - TIM barrel protein, at 2,596,577 to 2,597,356 _RS11705 PFLU_RS11710: PFLU_RS11710 - dehydrogenase, at 2,597,353 to 2,598,357 _RS11710 PFLU_RS11715: PFLU_RS11715 - sigma-54-dependent Fis family transcriptional regulator, at 2,598,544 to 2,600,505 _RS11715 PFLU_RS11720: PFLU_RS11720 - hypothetical protein, at 2,601,531 to 2,601,827 _RS11720 PFLU_RS11725: PFLU_RS11725 - LacI family transcriptional regulator, at 2,602,335 to 2,603,393 _RS11725
Group Condition PFLU_RS11705 PFLU_RS11710 PFLU_RS11715 PFLU_RS11720 PFLU_RS11725
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -1.1 -0.3 +0.2 -2.3 -0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.7 +0.0 -0.2 -1.6 -0.6
pH Growth at pH8 and (C) Trisodium citrate -1.0 +0.1 -0.0 -2.6 +0.6
carbon source Xanthosine (C) -0.7 -0.2 +0.5 -2.9 +0.3
carbon source caffeic 2 mM (C) -1.1 +0.6 +0.2 -1.7 -0.2
stress Betaine (C)(N); with MOPS; with chloride -0.8 -0.4 +0.0 -0.9 -0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -1.4 +0.0 -0.4 -0.4 +0.2
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -1.4 -0.7 -0.2 +0.8 +0.0
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.9 +0.1 -0.0 -1.2 +0.5
stress Betaine (C)(N); with MOPS; with chloride -1.0 -0.1 +0.2 -0.8 +0.2
carbon source L-Isoleucine (C) -0.5 +0.4 +0.1 -1.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.9 +0.0 -0.0 -2.2 -0.0
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.5 +0.1 +0.2 -1.9 -0.2
no stress control L-Glutamine (C)(N); with TAPS -1.1 +0.3 -0.2 +0.1 -0.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.9 -0.5 +0.0 -1.6 -0.1
temperature Growth at 25C +0.6 -0.9 +0.1 -0.7 +0.2
carbon source Phloretic Acid 2 mM (C) +0.4 -0.6 -0.3 +0.7 -1.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.3 -0.6 -0.3 +1.3 +0.5
pH Growth at pH9 and (C) Trisodium citrate +0.6 +0.8 +0.1 -1.3 -0.3
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.3 +0.4 +0.1 -1.2 +0.3
stress Betaine (C)(N); with TAPS; with chloride +0.9 -0.2 +0.1 -0.6 +0.3
no stress control L-Glutamine (C)(N); with MOPS +0.7 +0.6 -0.1 +0.4 -0.7
carbon source Xanthosine (C) -0.7 +0.2 +0.4 +1.4 -0.5
carbon source L-Valine 10 mM (C) +0.6 +0.7 +0.2 -0.5 +0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.7 +0.5 +0.3 +0.4 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +1.0 +0.3 +0.3 -0.3 +0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +1.1 +0.1 -0.2 +0.6 +0.2
carbon source 2-Deoxy-D-Ribose 10 mM (C) +0.8 +0.7 +0.3 +0.0 +0.3
no stress control Glycine betaine (C)(N); with TAPS +1.1 +0.1 +0.1 +0.6 +0.5
pH Growth at pH9 and (C) Trisodium citrate +0.5 +0.1 -0.1 +1.2 +0.8
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