Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS10945 and PFLU_RS10950 overlap by 4 nucleotidesPFLU_RS10950 and PFLU_RS10955 overlap by 4 nucleotidesPFLU_RS10955 and PFLU_RS10960 are separated by 58 nucleotidesPFLU_RS10960 and PFLU_RS10965 are separated by 256 nucleotides PFLU_RS10945: PFLU_RS10945 - ABC transporter substrate-binding protein, at 2,416,025 to 2,417,074 _RS10945 PFLU_RS10950: PFLU_RS10950 - homocysteine S-methyltransferase family protein, at 2,417,071 to 2,417,991 _RS10950 PFLU_RS10955: PFLU_RS10955 - peptide-methionine (S)-S-oxide reductase MsrA, at 2,417,988 to 2,418,455 _RS10955 PFLU_RS10960: PFLU_RS10960 - transcriptional regulator LrhA, at 2,418,514 to 2,419,374 _RS10960 PFLU_RS10965: PFLU_RS10965 - transporter substrate-binding domain-containing protein, at 2,419,631 to 2,420,476 _RS10965
Group Condition PFLU_RS10945 PFLU_RS10950 PFLU_RS10955 PFLU_RS10960 PFLU_RS10965
carbon source L-Carnitine (C) -0.2 -1.3 -0.8 -0.7 -0.4
stress Betaine (C)(N); with TAPS; with chloride -0.9 -0.7 -0.6 -0.7 -0.3
temperature Growth at 25C -0.1 +0.1 -2.8 +0.5 -0.8
carbon source caffeic 2 mM (C) +0.1 -0.4 -1.0 -0.1 -1.5
carbon source Shikimic Acid (C) -0.2 -0.6 -1.6 -1.1 +0.6
carbon source L-Carnitine (C) -0.3 +0.2 -2.6 -0.1 -0.1
carbon source propionate 20 mM (C) -0.3 -0.3 -0.9 -1.0 -0.3
pH Growth at pH9 and (C) Trisodium citrate -0.0 +0.1 -1.0 -0.3 -1.4
temperature Growth at 25C +0.2 -1.0 -1.0 -0.3 -0.3
pH Growth at pH9 and (C) D-Glucose +0.2 -0.4 -1.4 -0.0 -0.8
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.1 -0.2 -1.7 +0.2 -0.6
stress L-Glutamine (C)(N); with TAPS; with chloride +0.2 +0.3 -0.5 -1.3 -1.0
carbon source L-Valine (C) +0.8 +0.2 -0.8 -0.3 -1.9
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.1 -0.2 -0.2 +0.8 -2.1
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.0 -0.8 -0.8 +1.0 -1.1
stress Glucose (C) and Betaine (N); with TAPS; with chloride -0.3 -0.9 -0.4 -0.7 +0.7
nitrogen source NAG (N); with MOPS -0.2 +0.6 -0.2 -1.0 -0.7
carbon source L-Valine 10 mM (C) -0.3 -0.7 -0.9 -0.4 +0.9
carbon source 4-Aminobutyric (C) +0.5 +0.2 +0.1 -1.2 -0.6
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.2 -0.3 +1.0 -1.6 +0.3
stress Betaine (C)(N); with TAPS; with chloride +0.0 -0.6 -1.0 +0.1 +0.9
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.1 +0.5 +0.0 -2.1 +1.1
carbon source Shikimic Acid (C) -0.0 +0.5 +0.5 +0.7 -2.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.4 +0.5 -0.4 -0.8 +0.8
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.3 +0.7 +0.7 -1.3 -0.4
carbon source 4-Guanidinobutyric (C) -0.3 +0.7 +0.9 -0.3 -0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.1 +0.2 +0.6 -1.2 +0.5
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.6 +0.0 -0.3 +1.4 -0.3
carbon source p-Coumaric 2 mM (C) -0.1 -0.7 +0.6 -0.2 +1.5
carbon source D,L-Malic Acid (C) +0.5 +0.3 +0.8 +0.5 -0.7
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