Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS10935 and PFLU_RS30665 are separated by 14 nucleotidesPFLU_RS30665 and PFLU_RS10940 are separated by 287 nucleotidesPFLU_RS10940 and PFLU_RS10945 are separated by 14 nucleotidesPFLU_RS10945 and PFLU_RS10950 overlap by 4 nucleotides PFLU_RS10935: PFLU_RS10935 - alpha/beta hydrolase, at 2,412,614 to 2,413,489 _RS10935 PFLU_RS30665: PFLU_RS30665 - XRE family transcriptional regulator, at 2,413,504 to 2,413,626 _RS30665 PFLU_RS10940: PFLU_RS10940 - ATP-binding cassette domain-containing protein, at 2,413,914 to 2,416,010 _RS10940 PFLU_RS10945: PFLU_RS10945 - ABC transporter substrate-binding protein, at 2,416,025 to 2,417,074 _RS10945 PFLU_RS10950: PFLU_RS10950 - homocysteine S-methyltransferase family protein, at 2,417,071 to 2,417,991 _RS10950
Group Condition PFLU_RS10935 PFLU_RS30665 PFLU_RS10940 PFLU_RS10945 PFLU_RS10950
carbon source Phloretic Acid 2 mM (C) -0.9 N.D. -0.5 -0.1 -0.4
carbon source L-Carnitine (C) +0.1 N.D. -0.4 -0.2 -1.3
carbon source 4-Acetoxy-3-methoxycinnamic 2 mM (C) +0.0 N.D. -0.1 -0.2 -1.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 N.D. -0.5 -0.7 -0.8
stress Betaine (C)(N); with TAPS; with chloride -0.3 N.D. -0.6 +0.0 -0.6
nitrogen source NAG (N); with MOPS -0.7 N.D. -1.3 -0.2 +0.6
nitrogen source nitrate (N); with MOPS -0.1 N.D. -0.5 -0.3 -0.6
stress Glucose (C) and Betaine (N); with TAPS; with chloride -0.1 N.D. -0.2 -0.3 -0.9
carbon source D-Xylose (C) -0.4 N.D. -0.5 -0.1 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.5 N.D. -0.1 -0.5 -0.4
stress Betaine (C)(N); with TAPS; with chloride +0.0 N.D. +0.1 -0.9 -0.7
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.1 N.D. -1.1 -0.4 +0.5
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.2 N.D. -0.4 -0.5 -0.4
carbon source Xanthosine (C) -0.4 N.D. +0.3 -0.6 -0.2
pH Growth at pH9 and (C) Trisodium citrate -0.3 N.D. +0.3 -0.1 -0.6
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.3 N.D. +0.0 +0.1 +0.5
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.5 N.D. -0.5 -0.2 -0.5
temperature Growth at 25C +0.1 N.D. +0.1 +0.2 -1.0
carbon source NAG (C) +0.0 N.D. +0.6 -0.2 -1.0
carbon source 4-Guanidinobutyric (C) +0.3 N.D. -1.2 -0.3 +0.7
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.1 N.D. -0.6 -0.4 +0.5
stress L-Glutamine (C)(N); with TAPS; with chloride -0.3 N.D. -0.2 -0.4 +0.6
stress Betaine (C)(N); with MOPS; with chloride -0.1 N.D. -0.9 +0.2 +0.4
stress Betaine (C)(N); with TAPS; with chloride +0.3 N.D. +0.1 +0.2 -0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.1 N.D. -0.5 -0.2 +0.6
carbon source acetate (C) -0.3 N.D. +0.4 -0.1 +0.8
stress Betaine (C)(N); with MOPS; with chloride -0.5 N.D. +0.4 +0.3 +0.7
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.4 N.D. +0.4 -0.2 +0.6
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -0.1 N.D. +0.3 +0.3 +0.7
carbon source L-Valine (C) +0.0 N.D. +0.7 +0.8 +0.2
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