Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS10615 and PFLU_RS10620 are separated by 139 nucleotidesPFLU_RS10620 and PFLU_RS10625 are separated by 65 nucleotidesPFLU_RS10625 and PFLU_RS10630 are separated by 129 nucleotidesPFLU_RS10630 and PFLU_RS10635 are separated by 56 nucleotides PFLU_RS10615: PFLU_RS10615 - DDE-type integrase/transposase/recombinase, at 2,338,061 to 2,339,209 _RS10615 PFLU_RS10620: PFLU_RS10620 - MHS family MFS transporter, at 2,339,349 to 2,340,971 _RS10620 PFLU_RS10625: PFLU_RS10625 - choline dehydrogenase, at 2,341,037 to 2,342,683 _RS10625 PFLU_RS10630: PFLU_RS10630 - LysR family transcriptional regulator, at 2,342,813 to 2,343,706 _RS10630 PFLU_RS10635: PFLU_RS10635 - hypothetical protein, at 2,343,763 to 2,344,860 _RS10635
Group Condition PFLU_RS10615 PFLU_RS10620 PFLU_RS10625 PFLU_RS10630 PFLU_RS10635
carbon source L-Valine 10 mM (C) N.D. -0.8 -0.3 -1.2 -0.4
carbon source L-Valine 10 mM (C) N.D. -0.5 -0.3 -1.0 -0.1
carbon source L-Valine (C) N.D. -0.4 -0.3 -1.4 +0.3
carbon source L-Valine (C) N.D. -0.8 +0.1 -0.4 -0.6
temperature Growth at 25C N.D. -0.3 -0.3 -0.6 -0.4
stress Betaine (C)(N); with MOPS; with chloride N.D. -0.2 -0.1 -0.8 -0.3
stress L-Glutamine (C)(N); with TAPS; with chloride N.D. -0.4 -0.4 -0.4 -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride N.D. +0.1 -0.4 -0.5 -0.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride N.D. +0.2 -0.2 -0.8 -0.3
stress Betaine (C)(N); with MOPS; with chloride N.D. +0.2 +0.1 -0.9 -0.3
carbon source L-Valine 10 mM (C) N.D. -0.5 -0.7 +0.2 +0.2
no stress control L-Glutamine (C)(N); with TAPS N.D. +0.1 +0.3 -1.3 +0.2
carbon source Shikimic Acid (C) N.D. +0.0 +0.4 -0.6 -0.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride N.D. +0.1 -0.3 +0.4 -0.8
pH Growth at pH9 and (C) Trisodium citrate N.D. -0.4 -0.5 +0.6 -0.0
carbon source propionate (C) N.D. +0.3 +0.1 -0.8 +0.1
pH Growth at pH8 and (C) D-Glucose N.D. -0.2 +0.3 +0.5 -0.6
carbon and nitrogen source NAG carbon and (N) N.D. -0.0 -0.6 +0.7 -0.1
carbon source Phloretic Acid 2 mM (C) N.D. -0.0 +0.3 -0.7 +0.6
stress Betaine (C)(N); with MOPS; with chloride N.D. +0.2 -0.2 +0.5 -0.4
no stress control Glycine betaine (C)(N); with TAPS N.D. +0.3 -0.4 +0.7 -0.1
carbon source Xanthosine (C) N.D. +0.2 +0.6 +0.3 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +0.2 +0.4 -0.3 +0.6
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride N.D. -0.3 +0.4 +0.9 +0.2
carbon source Inosine (C) N.D. +0.2 -0.0 +0.8 +0.4
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS N.D. +0.3 +0.2 +0.8 +0.0
carbon source pyruvate 20 mM (C) N.D. -0.2 +0.5 +1.1 +0.2
carbon source D,L-Malic Acid (C) N.D. -0.0 +0.3 +1.0 +0.3
carbon source pyruvate 20 mM (C) N.D. -0.0 +0.5 +1.3 -0.2
carbon source pyruvate 20 mM (C) N.D. -0.1 +0.6 +1.3 -0.0
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