Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS10520 and PFLU_RS10525 are separated by 228 nucleotidesPFLU_RS10525 and PFLU_RS10530 are separated by 127 nucleotidesPFLU_RS10530 and PFLU_RS10535 are separated by 134 nucleotidesPFLU_RS10535 and PFLU_RS10540 are separated by 28 nucleotides PFLU_RS10520: PFLU_RS10520 - serine/threonine transporter SstT, at 2,318,583 to 2,319,803 _RS10520 PFLU_RS10525: PFLU_RS10525 - MFS transporter, at 2,320,032 to 2,321,186 _RS10525 PFLU_RS10530: PFLU_RS10530 - transcriptional activator NhaR, at 2,321,314 to 2,322,216 _RS10530 PFLU_RS10535: PFLU_RS10535 - hypothetical protein, at 2,322,351 to 2,322,602 _RS10535 PFLU_RS10540: PFLU_RS10540 - TerC family protein, at 2,322,631 to 2,323,380 _RS10540
Group Condition PFLU_RS10520 PFLU_RS10525 PFLU_RS10530 PFLU_RS10535 PFLU_RS10540
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.2 +0.0 -1.9 N.D. -0.2
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.4 -0.8 -0.3 N.D. -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 +0.1 -1.5 N.D. -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.1 +0.1 -1.0 N.D. -0.4
carbon source D-Glucose (C) 5 mM +0.2 -0.2 -0.8 N.D. -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 -0.3 -0.7 N.D. -0.1
pH Growth at pH9 and (C) Trisodium citrate +0.3 +0.2 -0.5 N.D. -0.6
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.4 -0.9 +0.1 N.D. -0.2
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -0.4 +0.4 -0.6 N.D. +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.0 -0.3 -0.7 N.D. +0.5
carbon source NAG (C) +0.6 -0.6 -0.3 N.D. -0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.5 -0.1 -0.9 N.D. +0.1
pH Growth at pH7 and (C) D-Glucose -0.5 -0.5 +0.1 N.D. +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.2 +0.6 -0.4 N.D. -0.3
no stress control Glycine betaine (C)(N); with MOPS -0.4 -0.6 +0.8 N.D. +0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.9 -0.6 -0.3 N.D. -0.0
stress Betaine (C)(N); with TAPS; with chloride -0.2 -0.4 +0.9 N.D. -0.2
carbon source L-Valine (C) +0.4 +0.7 -1.0 N.D. +0.2
carbon source pyruvate 20 mM (C) +0.2 -0.2 +0.8 N.D. -0.6
stress L-Glutamine (C)(N); with TAPS; with chloride -0.1 +0.3 +0.7 N.D. -0.6
carbon source 4-Acetoxy-3-methoxycinnamic 2 mM (C) +0.2 -0.4 +0.8 N.D. -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.1 +0.4 +0.5 N.D. -0.5
no stress control L-Glutamine (C)(N); with TAPS +0.2 +0.4 +0.5 N.D. -0.5
carbon source D,L-Malic Acid (C) +0.8 -0.5 +0.5 N.D. +0.0
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.7 +0.3 -0.3 N.D. +0.3
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.8 +0.2 +0.7 N.D. -0.5
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.6 +0.3 +0.5 N.D. -0.1
carbon source L-Valine 10 mM (C) -0.1 +0.9 +0.4 N.D. +0.2
pH Growth at pH9 and (C) Trisodium citrate +1.0 -0.0 -0.0 N.D. +0.5
carbon source L-Valine 10 mM (C) -0.0 +0.8 +0.7 N.D. +0.4
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