Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS30610 and PFLU_RS09905 are separated by 61 nucleotidesPFLU_RS09905 and PFLU_RS09910 are separated by 166 nucleotidesPFLU_RS09910 and PFLU_RS09915 are separated by 110 nucleotidesPFLU_RS09915 and PFLU_RS31515 overlap by 29 nucleotides PFLU_RS30610: PFLU_RS30610 - IS3 family transposase, at 2,184,011 to 2,184,361 _RS30610 PFLU_RS09905: PFLU_RS09905 - LysR family transcriptional regulator, at 2,184,423 to 2,185,331 _RS09905 PFLU_RS09910: PFLU_RS09910 - MFS transporter, at 2,185,498 to 2,186,796 _RS09910 PFLU_RS09915: PFLU_RS09915 - M81 family metallopeptidase, at 2,186,907 to 2,188,451 _RS09915 PFLU_RS31515: PFLU_RS31515 - Lrp/AsnC family transcriptional regulator, at 2,188,423 to 2,188,524 _RS31515
Group Condition PFLU_RS30610 PFLU_RS09905 PFLU_RS09910 PFLU_RS09915 PFLU_RS31515
temperature shift Temperature shift 10_to_25; with MOPS -2.2 -0.5 +0.3 -0.2 N.D.
stress Betaine (C)(N); with TAPS; with chloride -2.8 +0.2 +0.0 +0.2 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.7 -0.2 -0.4 +0.1 N.D.
carbon source Shikimic Acid (C) -1.4 -0.3 -0.1 -0.2 N.D.
stress Betaine (C)(N); with TAPS; with chloride -1.6 -0.2 -0.2 -0.0 N.D.
carbon source Xanthosine (C) -2.0 -0.0 +0.1 -0.1 N.D.
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.9 -0.6 -0.4 -0.0 N.D.
carbon source D-Glucose (C); with MOPS -1.5 -0.1 -0.0 -0.3 N.D.
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.7 -0.4 -0.4 -0.4 N.D.
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.7 -0.6 -0.2 -0.3 N.D.
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -1.5 -0.2 +0.0 -0.1 N.D.
carbon source p-Coumaric (C) 5 mM -1.3 +0.4 -0.7 +0.0 N.D.
carbon source Choline chloride 10 mM (C) -1.5 +0.2 -0.3 -0.0 N.D.
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -1.1 -0.2 -0.4 +0.1 N.D.
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -1.3 -0.2 -0.1 +0.1 N.D.
stress Betaine (C)(N); with TAPS; with chloride -1.0 +0.2 -0.4 -0.1 N.D.
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.3 -0.5 -0.7 -0.4 N.D.
carbon source Vanillic Acid (C) -1.6 +0.0 +0.0 +0.1 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.2 -0.3 +0.2 -0.0 N.D.
nitrogen source NAG (N) -1.1 +0.2 -0.1 -0.2 N.D.
no stress control Glycine betaine (C)(N); with MOPS -1.6 +0.4 -0.1 +0.1 N.D.
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -1.2 +0.3 -0.0 -0.3 N.D.
carbon source Fumarate 20 mM (C) -1.2 -0.2 +0.2 +0.1 N.D.
carbon source 4-Guanidinobutyric (C) -0.5 -0.6 -0.3 +0.3 N.D.
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS -1.5 +0.2 +0.2 -0.0 N.D.
carbon source D-Glucose (C) 5 mM -1.4 +0.1 +0.1 +0.1 N.D.
pH Growth at pH9 and (C) D-Glucose -1.0 +0.5 -0.3 -0.1 N.D.
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.2 -0.5 -0.5 +0.4 N.D.
carbon source L-Valine 10 mM (C) +1.0 -0.4 -0.5 -0.0 N.D.
no stress control Glycine betaine (C)(N); with TAPS +0.6 +0.4 -0.4 +0.3 N.D.
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