Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS31510 and PFLU_RS30610 are separated by 164 nucleotidesPFLU_RS30610 and PFLU_RS09905 are separated by 61 nucleotidesPFLU_RS09905 and PFLU_RS09910 are separated by 166 nucleotidesPFLU_RS09910 and PFLU_RS09915 are separated by 110 nucleotides PFLU_RS31510: PFLU_RS31510 - transcriptional regulator, at 2,183,760 to 2,183,846 _RS31510 PFLU_RS30610: PFLU_RS30610 - IS3 family transposase, at 2,184,011 to 2,184,361 _RS30610 PFLU_RS09905: PFLU_RS09905 - LysR family transcriptional regulator, at 2,184,423 to 2,185,331 _RS09905 PFLU_RS09910: PFLU_RS09910 - MFS transporter, at 2,185,498 to 2,186,796 _RS09910 PFLU_RS09915: PFLU_RS09915 - M81 family metallopeptidase, at 2,186,907 to 2,188,451 _RS09915
Group Condition PFLU_RS31510 PFLU_RS30610 PFLU_RS09905 PFLU_RS09910 PFLU_RS09915
no stress control Glycine betaine (C)(N); with TAPS -5.7 +0.6 +0.4 -0.4 +0.3
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS -4.8 +0.1 +0.2 +0.2 +0.1
stress Betaine (C)(N); with TAPS; with chloride -2.4 -0.7 -0.5 -0.1 -0.1
carbon source Phloretic Acid 2 mM (C) -3.8 +0.4 -0.4 -0.0 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -2.1 -1.1 -0.2 -0.4 +0.1
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS -4.6 +0.3 +0.5 +0.3 -0.1
carbon source Xanthosine (C) -3.5 -0.4 +0.0 +0.2 +0.2
carbon source Trisodium citrate (C) -3.1 +0.1 -0.1 -0.2 -0.2
stress Betaine (C)(N); with MOPS; with chloride -2.8 -0.0 -0.2 -0.2 -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -3.4 -0.2 +0.0 +0.1 +0.2
temperature Growth at 30C -2.7 -1.0 +0.3 +0.0 +0.1
carbon source Shikimic Acid (C) -1.2 -1.4 -0.3 -0.1 -0.2
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -1.8 -0.3 -1.0 -0.0 -0.1
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -2.2 -0.2 -0.3 -0.6 +0.1
stress Betaine (C)(N); with TAPS; with chloride -0.5 -2.8 +0.2 +0.0 +0.2
carbon source Xanthosine (C) -0.8 -2.0 -0.0 +0.1 -0.1
pH Growth at pH9 and (C) D-Glucose -1.7 -1.0 +0.5 -0.3 -0.1
carbon source L-Ornithine 10 mM (C) -3.1 +0.3 -0.1 +0.4 +0.3
temperature shift Temperature shift 10_to_25; with MOPS +0.4 -2.2 -0.5 +0.3 -0.2
carbon source 2-Deoxy-D-Ribose 10 mM (C) -2.5 +0.8 -0.1 +0.3 -0.0
carbon source p-Coumaric (C) 5 mM +0.9 -1.3 +0.4 -0.7 +0.0
carbon source 4-Aminobutyric (C) -2.0 +0.9 +0.3 +0.3 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +1.3 -0.9 -0.6 -0.4 -0.0
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +1.7 -1.5 -0.2 +0.0 -0.1
stress Betaine (C)(N); with MOPS; with chloride +2.1 -0.4 -0.6 +0.3 +0.1
carbon source L-Valine (C) +3.2 -0.3 -1.1 -0.1 +0.0
carbon source L-Valine 10 mM (C) +1.6 +1.0 -0.4 -0.5 -0.0
carbon source L-Valine 10 mM (C) +1.7 -0.7 +0.8 +0.1 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +2.2 +0.8 -0.2 +0.3 -0.1
carbon source L-Valine (C) +3.1 -0.2 -0.1 +0.5 -0.1
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