Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS05290 and PFLU_RS05295 overlap by 4 nucleotidesPFLU_RS05295 and PFLU_RS05300 are separated by 185 nucleotidesPFLU_RS05300 and PFLU_RS05305 are separated by 1 nucleotidesPFLU_RS05305 and PFLU_RS05310 are separated by 208 nucleotides PFLU_RS05290: PFLU_RS05290 - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 1,178,738 to 1,179,694 _RS05290 PFLU_RS05295: PFLU_RS05295 - carboxy-S-adenosyl-L-methionine synthase CmoA, at 1,179,691 to 1,180,434 _RS05295 PFLU_RS05300: PFLU_RS05300 - lysoplasmalogenase, at 1,180,620 to 1,181,255 _RS05300 PFLU_RS05305: PFLU_RS05305 - protease inhibitor I42 family protein, at 1,181,257 to 1,181,646 _RS05305 PFLU_RS05310: PFLU_RS05310 - endopeptidase La, at 1,181,855 to 1,184,275 _RS05310
Group Condition PFLU_RS05290 PFLU_RS05295 PFLU_RS05300 PFLU_RS05305 PFLU_RS05310
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.5 -1.4 -0.2 -1.0 -0.6
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -0.8 -0.7 -0.4 -1.3 -0.4
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -0.7 -1.1 -0.8 -0.8 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.6 -0.5 -0.3 -1.4 -0.5
carbon source acetate 30 mM (C) -1.6 -2.1 +0.4 -0.2 +0.3
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.2 -0.7 -1.2 -1.3 +0.3
carbon source acetate 30 mM (C) -1.5 -1.7 +0.5 -0.6 +0.4
stress Betaine (C)(N); with MOPS; with chloride -0.4 -0.7 -0.6 -0.9 -0.1
carbon source Choline chloride 10 mM (C) -0.2 -0.3 -0.1 -2.1 -0.2
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -0.1 -0.0 -0.8 -1.7 -0.1
stress malate (C) and Ammonium chloride (N); with TAPS -0.0 -0.2 -0.6 -1.2 -0.6
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -0.5 -0.4 -0.3 -0.9 -0.4
carbon source L-Carnitine 10 mM (C) -0.6 -1.4 -0.5 -0.1 +0.1
carbon source 2-Deoxy-D-Ribose 10 mM (C) -0.2 -0.4 -0.6 -1.3 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.3 -1.3 -0.1 +0.2 -0.8
carbon source Phloretic Acid 2 mM (C) -0.4 -2.1 +0.7 +0.0 -0.4
no stress control Glycine betaine (C)(N); with MOPS -0.7 -1.3 +0.6 -0.5 -0.4
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -1.0 -0.8 -0.0 -0.6 +0.3
carbon source Phloretic Acid 2 mM (C) -0.7 -0.6 -0.8 +0.3 -0.4
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -0.7 -0.8 +0.1 -0.7 +0.2
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -0.8 -0.6 +0.4 -1.0 +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.7 -0.4 -0.9 +0.5 -0.3
pH Growth at pH9 and (C) Trisodium citrate -0.5 -1.5 +0.2 -0.1 +0.5
stress Betaine (C)(N); with TAPS; with chloride -0.2 -1.8 +0.2 +0.9 -0.5
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.3 -0.4 +0.8 -0.7 -0.4
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.2 +0.6 -0.2 -1.5 +0.0
nitrogen source NAG (N); with MOPS -0.3 -0.8 +0.8 -0.2 -0.4
pH Growth at pH8 and (C) Trisodium citrate -0.4 -2.1 +0.6 +0.9 +0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.2 +0.8 -0.5 +1.2 -0.4
carbon source L-Valine 10 mM (C) +0.3 +0.3 +0.4 +0.9 -0.8
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