Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS04730 and PFLU_RS04735 are separated by 112 nucleotidesPFLU_RS04735 and PFLU_RS04740 are separated by 363 nucleotidesPFLU_RS04740 and PFLU_RS30220 overlap by 8 nucleotidesPFLU_RS30220 and PFLU_RS04750 are separated by 68 nucleotides PFLU_RS04730: PFLU_RS04730 - cell division protein FtsZ, at 1,057,136 to 1,058,341 _RS04730 PFLU_RS04735: PFLU_RS04735 - UDP-3-O-acyl-N-acetylglucosamine deacetylase, at 1,058,454 to 1,059,365 _RS04735 PFLU_RS04740: PFLU_RS04740 - hypothetical protein, at 1,059,729 to 1,060,202 _RS04740 PFLU_RS30220: PFLU_RS30220 - hypothetical protein, at 1,060,195 to 1,061,199 _RS30220 PFLU_RS04750: PFLU_RS04750 - GGDEF domain-containing protein, at 1,061,268 to 1,062,761 _RS04750
Group Condition PFLU_RS04730 PFLU_RS04735 PFLU_RS04740 PFLU_RS30220 PFLU_RS04750
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride N.D. N.D. -1.5 -0.5 -0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride N.D. N.D. -2.0 +0.1 -0.2
carbon source L-Carnitine (C) N.D. N.D. -2.0 +0.0 +0.1
pH Growth at pH9 and (C) Trisodium citrate N.D. N.D. -1.8 +0.2 -0.0
carbon source 4-Aminobutyric (C) N.D. N.D. -1.4 -0.1 -0.1
stress Betaine (C)(N); with MOPS; with chloride N.D. N.D. -0.9 -0.2 -0.4
carbon source L-Valine 10 mM (C) N.D. N.D. -0.9 -0.2 -0.4
carbon source propionate 20 mM (C) N.D. N.D. -0.3 -0.5 -0.6
carbon source L-tyrosine 10 mM (C) N.D. N.D. -1.3 -0.1 +0.0
no stress control Glycine betaine (C)(N); with TAPS N.D. N.D. -1.3 -0.1 +0.0
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS N.D. N.D. -1.5 -0.1 +0.3
no stress control Glycine betaine (C)(N); with MOPS N.D. N.D. -1.3 -0.1 +0.1
carbon source 4-Hydroxybenzoic Acid (C) 2.5 mM N.D. N.D. -1.4 +0.1 +0.1
carbon source D,L-Malic Acid (C) N.D. N.D. -1.5 +0.2 +0.1
nitrogen source no extra (N); with MOPS N.D. N.D. -1.5 +0.1 +0.3
temperature shift Temperature shift 10_to_25; with MOPS N.D. N.D. -0.7 -0.5 +0.1
temperature shift Temperature shift 10_to_25 N.D. N.D. -1.1 +0.1 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. N.D. -1.1 +0.3 -0.0
carbon source 4-Aminobutyric (C) N.D. N.D. -0.4 +0.3 -0.7
stress Betaine (C)(N); with TAPS; with chloride N.D. N.D. -0.8 -0.3 +0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride N.D. N.D. +0.6 -0.6 -0.4
carbon source Xanthosine (C) N.D. N.D. -0.5 -0.3 +0.6
no stress control L-Glutamine (C)(N); with TAPS N.D. N.D. +0.6 -0.6 -0.2
carbon source p-Coumaric 2 mM (C) N.D. N.D. +0.7 -0.6 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. N.D. -0.5 +0.8 +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride N.D. N.D. +1.0 +0.1 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. N.D. +1.1 +0.2 -0.2
carbon source Inosine (C) N.D. N.D. +1.2 -0.1 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. N.D. +0.8 -0.1 +0.6
stress Betaine (C)(N); with TAPS; with chloride N.D. N.D. +1.0 +0.1 +0.4
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