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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens SBW25-INTG
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PFLU_RS04070 and PFLU_RS04075 are separated by 259 nucleotides
PFLU_RS04075 and PFLU_RS04080 are separated by 98 nucleotides
PFLU_RS04080 and PFLU_RS04085 are separated by 225 nucleotides
PFLU_RS04085 and PFLU_RS04090 overlap by 1 nucleotides
PFLU_RS04070: PFLU_RS04070 - peptide chain release factor 3, at 922,396 to 923,979
_RS04070
PFLU_RS04075: PFLU_RS04075 - hypothetical protein, at 924,239 to 924,484
_RS04075
PFLU_RS04080: PFLU_RS04080 - hypothetical protein, at 924,583 to 924,846
_RS04080
PFLU_RS04085: PFLU_RS04085 - ABC transporter ATP-binding protein, at 925,072 to 926,043
_RS04085
PFLU_RS04090: PFLU_RS04090 - ABC transporter ATP-binding protein, at 926,043 to 927,011
_RS04090
Group
Condition
PFLU
_RS04070
PFLU
_RS04075
PFLU
_RS04080
PFLU
_RS04085
PFLU
_RS04090
carbon source
L-Arabinose (C); with MOPS
-3.7
-2.8
+0.2
-0.5
-0.4
nitrogen source
NAG (N); with MOPS
-3.4
-2.9
-0.1
-0.1
-0.2
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-2.5
-3.7
-0.4
+0.0
-0.1
nitrogen source
NAG (N); with MOPS
-3.3
-2.1
-0.5
+0.1
-0.1
no stress control
Glycine betaine (C)(N); with TAPS
-2.0
-3.7
-0.1
-0.1
+0.0
carbon source
L-Arabinose (C); with MOPS
-3.4
-1.0
-0.5
-0.0
-0.7
carbon and nitrogen source
NAG carbon and (N)
-3.2
-2.8
+0.4
+0.4
-0.2
stress
Sucrose (C) and Ammonium chloride (N); with TAPS; with PEG
-2.7
-1.9
-0.1
+0.0
-0.5
carbon source
NAG (C)
-2.9
-2.8
+0.1
+0.1
+0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-2.2
-2.1
-0.8
+0.2
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-1.8
-2.9
-0.4
+0.1
+0.1
nitrogen source
Urea (N); with MOPS
-3.1
-1.6
+0.1
-0.2
+0.1
pH
Growth at pH8 and (C) Trisodium citrate
-2.6
-2.7
+0.6
+0.5
-0.4
no stress control
Glycine betaine (C)(N); with MOPS
-1.1
-3.6
+0.1
-0.3
+0.4
no stress control
Glucose (C) and Glycine betaine (N); with TAPS
-1.7
-2.5
+0.7
-0.2
-0.2
stress
Sucrose (C) and Ammonium chloride (N); with TAPS
-2.7
-2.0
-0.0
+0.2
+0.6
no stress control
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-1.0
-3.6
+0.2
-0.0
+0.7
carbon and nitrogen source
NAG carbon and (N); with MOPS
-3.6
+1.3
-0.2
-0.3
-0.9
no stress control
Growth with no stress
-3.5
+0.8
-0.0
-0.2
-0.6
nitrogen source
nitrate (N)
-3.7
+1.0
-0.4
-0.0
-0.4
carbon source
L-Arabinose (C)
-3.9
+1.5
-0.1
-0.3
-0.7
temperature
Growth at 10C; with MOPS
-3.7
+0.9
+0.0
-0.2
-0.3
nitrogen source
no extra (N); with MOPS
-3.5
+0.6
+0.4
+0.0
-0.7
stress
Betaine (C)(N); with MOPS; with chloride
-0.3
-2.6
-1.1
-0.1
+0.9
carbon and nitrogen source
NAG carbon and (N); with MOPS
-3.5
+1.4
+0.3
-0.1
+0.3
pH
Growth at pH9 and (C) Trisodium citrate
-2.7
+2.7
-0.0
-0.4
-0.1
carbon source
p-Coumaric (C) 5 mM
+1.1
-2.9
-0.1
+0.8
+0.5
carbon source
L-Valine (C)
+1.2
-2.4
-0.2
+0.2
+1.1
carbon source
D-Glucosamine Hydrochloride 10 mM (C)
+2.7
+1.1
-1.0
-0.3
-0.3
stress
Betaine (C)(N); with MOPS; with chloride
-0.3
+2.9
+0.7
-0.4
+0.8
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