Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS03895 and PFLU_RS03900 are separated by 94 nucleotidesPFLU_RS03900 and PFLU_RS03905 are separated by 132 nucleotidesPFLU_RS03905 and PFLU_RS03910 are separated by 60 nucleotidesPFLU_RS03910 and PFLU_RS03915 are separated by 54 nucleotides PFLU_RS03895: PFLU_RS03895 - tRNA-Lys, at 885,528 to 885,603 _RS03895 PFLU_RS03900: PFLU_RS03900 - tRNA-Pro, at 885,698 to 885,774 _RS03900 PFLU_RS03905: PFLU_RS03905 - NAD(P)/FAD-dependent oxidoreductase, at 885,907 to 887,205 _RS03905 PFLU_RS03910: PFLU_RS03910 - DUF3094 family protein, at 887,266 to 887,439 _RS03910 PFLU_RS03915: PFLU_RS03915 - DUF1780 domain-containing protein, at 887,494 to 888,123 _RS03915
Group Condition PFLU_RS03895 PFLU_RS03900 PFLU_RS03905 PFLU_RS03910 PFLU_RS03915
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS N.D. N.D. +0.0 -1.4 -0.7
nitrogen source Ammonium chloride (N); with MOPS N.D. N.D. +0.1 -2.2 +0.3
no stress control Glycine betaine (C)(N); with TAPS N.D. N.D. +0.1 -1.5 -0.4
carbon source L-Valine 10 mM (C) N.D. N.D. -0.4 -0.7 -0.6
carbon source L-Valine (C) N.D. N.D. -0.5 -0.1 -0.8
temperature shift Temperature shift 10_to_25; with MOPS N.D. N.D. +0.1 -0.8 -0.6
no stress control L-Glutamine (C)(N); with MOPS N.D. N.D. -0.2 -1.2 +0.1
carbon source p-Coumaric (C) N.D. N.D. -0.2 -0.7 -0.4
pH Growth at pH9 and (C) Trisodium citrate N.D. N.D. -0.6 -1.0 +0.4
stress Betaine (C)(N); with MOPS; with chloride N.D. N.D. +0.0 -0.7 -0.6
carbon source L-tyrosine 10 mM (C) N.D. N.D. -0.2 -1.1 +0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride N.D. N.D. -0.0 -1.1 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. N.D. +0.0 -0.8 -0.4
carbon source propionate 20 mM (C) N.D. N.D. -0.2 -0.7 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. N.D. +0.3 -1.0 -0.5
carbon source 4-hydroxyphenylacetic 2 mM (C) N.D. N.D. +0.3 -0.9 -0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride N.D. N.D. -0.7 +0.4 -0.3
no stress control Glycine betaine (C)(N); with TAPS N.D. N.D. +0.4 -0.2 -0.7
stress L-Glutamine (C)(N); with MOPS; with chloride N.D. N.D. -0.2 +0.7 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. N.D. +0.2 -0.7 +0.3
stress Betaine (C)(N); with TAPS; with chloride N.D. N.D. +0.2 -0.6 +0.5
carbon source L-Ornithine 10 mM (C) N.D. N.D. +0.3 +0.4 -0.6
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS N.D. N.D. -0.3 +0.8 -0.3
carbon source acetate 30 mM (C) N.D. N.D. +0.2 +0.7 -0.5
carbon source 4-hydroxyphenylacetic 2 mM (C) N.D. N.D. +0.2 +0.7 -0.4
carbon source p-Coumaric 2 mM (C) N.D. N.D. -0.3 +1.0 -0.0
carbon source p-Coumaric (C) 5 mM N.D. N.D. -0.0 +0.9 +0.4
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS N.D. N.D. +0.2 +0.6 +0.4
pH Growth at pH7 and (C) D-Glucose N.D. N.D. +0.1 +0.7 +0.6
carbon source Ferulic Acid (C) 1.5 mM N.D. N.D. -0.2 +1.6 +0.5
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