Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS03815 and PFLU_RS03820 overlap by 8 nucleotidesPFLU_RS03820 and PFLU_RS03825 are separated by 181 nucleotidesPFLU_RS03825 and PFLU_RS03830 overlap by 47 nucleotidesPFLU_RS03830 and PFLU_RS03835 are separated by 193 nucleotides PFLU_RS03815: PFLU_RS03815 - lipoprotein signal peptidase, at 872,992 to 873,504 _RS03815 PFLU_RS03820: PFLU_RS03820 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 873,497 to 873,949 _RS03820 PFLU_RS03825: PFLU_RS03825 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 874,131 to 875,078 _RS03825 PFLU_RS03830: PFLU_RS03830 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 875,032 to 875,613 _RS03830 PFLU_RS03835: PFLU_RS03835 - type IV pilus modification protein PilV, at 875,807 to 876,292 _RS03835
Group Condition PFLU_RS03815 PFLU_RS03820 PFLU_RS03825 PFLU_RS03830 PFLU_RS03835
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS N.D. N.D. N.D. -1.8 -1.0
carbon source Shikimic Acid (C) N.D. N.D. N.D. -2.5 -0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride N.D. N.D. N.D. -1.8 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. N.D. N.D. -1.3 -0.8
carbon source L-Carnitine (C) N.D. N.D. N.D. -2.1 +0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride N.D. N.D. N.D. -1.8 +0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride N.D. N.D. N.D. -1.7 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. N.D. N.D. -1.4 -0.2
carbon source pyruvate 20 mM (C) N.D. N.D. N.D. -1.8 +0.1
stress L-Glutamine (C)(N); with TAPS; with chloride N.D. N.D. N.D. -1.6 +0.0
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS N.D. N.D. N.D. -2.0 +0.5
carbon source caffeic 2 mM (C) N.D. N.D. N.D. -0.5 -1.0
stress Betaine (C)(N); with TAPS; with chloride N.D. N.D. N.D. -0.1 -1.3
carbon source L-Isoleucine (C) N.D. N.D. N.D. -1.4 +0.2
no stress control L-Glutamine (C)(N); with MOPS N.D. N.D. N.D. -1.3 +0.2
carbon source Inosine 10 mM (C) N.D. N.D. N.D. -1.2 +0.3
no stress control Sucrose (C) and Ammonium chloride (N); with TAPS N.D. N.D. N.D. -1.2 +0.2
carbon source 4-Hydroxybenzoic Acid 2 mM (C) N.D. N.D. N.D. -1.1 +0.4
carbon source propionate 20 mM (C) N.D. N.D. N.D. -1.0 +0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride N.D. N.D. N.D. -1.0 +0.4
carbon source L-Valine (C) N.D. N.D. N.D. +0.5 -1.0
pH Growth at pH6 and (C) D-Glucose N.D. N.D. N.D. +0.6 -1.0
pH Growth at pH8 and (C) Trisodium citrate N.D. N.D. N.D. +0.8 -0.8
pH Growth at pH8 and (C) D-Glucose N.D. N.D. N.D. +0.9 -0.6
carbon source acetate 30 mM (C) N.D. N.D. N.D. +0.9 -0.5
temperature Growth at 25C; with MOPS N.D. N.D. N.D. +1.1 -0.5
temperature Growth at 25C N.D. N.D. N.D. +1.3 -0.5
carbon source Inosine (C) N.D. N.D. N.D. +1.3 -0.2
nitrogen source nitrate (N); with MOPS N.D. N.D. N.D. +1.0 +0.4
pH Growth at pH9 and (C) Trisodium citrate N.D. N.D. N.D. +1.5 +0.0
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