Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS03455 and PFLU_RS03460 are separated by 109 nucleotidesPFLU_RS03460 and PFLU_RS03465 are separated by 18 nucleotidesPFLU_RS03465 and PFLU_RS03470 are separated by 18 nucleotidesPFLU_RS03470 and PFLU_RS03475 overlap by 86 nucleotides PFLU_RS03455: PFLU_RS03455 - cation transporter, at 795,624 to 796,529 _RS03455 PFLU_RS03460: PFLU_RS03460 - TolC family protein, at 796,639 to 797,862 _RS03460 PFLU_RS03465: PFLU_RS03465 - efflux RND transporter periplasmic adaptor subunit, at 797,881 to 798,987 _RS03465 PFLU_RS03470: PFLU_RS03470 - CusA/CzcA family heavy metal efflux RND transporter, at 799,006 to 802,152 _RS03470 PFLU_RS03475: PFLU_RS03475 - heavy metal sensor histidine kinase, at 802,067 to 803,503 _RS03475
Group Condition PFLU_RS03455 PFLU_RS03460 PFLU_RS03465 PFLU_RS03470 PFLU_RS03475
carbon source L-Valine (C) +0.0 -0.8 -0.7 -0.3 -0.4
carbon source Phloretic Acid 2 mM (C) -0.4 -0.4 -0.2 -0.3 -0.5
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.3 -0.2 -0.8 -0.4 +0.0
carbon source p-Coumaric 2 mM (C) -0.2 -0.4 -0.7 -0.0 -0.2
carbon source L-Lysine (C) -0.2 -0.4 -0.3 -0.0 -0.6
nitrogen source NAG (N); with MOPS -0.3 -0.2 -0.7 -0.2 +0.0
pH Growth at pH9 and (C) Trisodium citrate -0.7 -0.7 -0.2 +0.1 +0.2
carbon source NAG (C) -0.3 -0.9 -0.2 -0.1 +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.4 -0.6 -0.3 -0.1 +0.1
carbon source 2-Deoxy-D-Ribose 10 mM (C) +0.3 +0.0 -0.8 +0.1 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.2 -0.6 -0.4 +0.1 +0.2
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS +0.2 -0.3 -0.6 -0.0 -0.1
carbon source 4-Guanidinobutyric (C) +0.2 -0.5 -0.8 +0.3 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.6 -0.3 +0.2 +0.1 -0.0
carbon source sn-glycero-3-phosphocholine 10 mM (C) -0.2 +0.4 -0.4 +0.1 -0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.6 -0.1 +0.1 -0.3 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 -0.4 +0.3 -0.3 +0.4
carbon source propionate 20 mM (C) -0.4 +0.7 -0.3 -0.0 +0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.2 +0.2 -0.4 +0.3 -0.4
carbon source Phloretic Acid 2 mM (C) -0.0 -0.0 -0.6 +0.5 +0.2
carbon source p-Coumaric (C) 5 mM +0.1 +0.6 +0.1 -0.1 -0.7
pH Growth at pH6 and (C) D-Glucose +0.2 +0.2 -0.4 +0.2 -0.2
carbon source 2-Deoxy-D-Ribose 10 mM (C) -0.3 -0.3 +0.5 +0.2 +0.1
stress Betaine (C)(N); with MOPS; with chloride +0.1 +0.3 -0.4 -0.2 +0.4
carbon source L-Valine (C) -0.2 +0.2 +0.1 +0.4 -0.3
no stress control Growth with no stress; with MOPS +0.2 -0.3 -0.2 +0.3 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 -0.5 +0.5 +0.3 -0.1
carbon source L-Carnitine (C) +0.5 -0.2 +0.0 +0.2 +0.4
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.2 +0.6 -0.1 +0.1 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.4 +0.4 +0.5 +0.1 +0.1
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