Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS03250 and PFLU_RS03255 are separated by 85 nucleotidesPFLU_RS03255 and PFLU_RS03260 are separated by 8 nucleotidesPFLU_RS03260 and PFLU_RS03265 are separated by 146 nucleotidesPFLU_RS03265 and PFLU_RS03270 are separated by 24 nucleotides PFLU_RS03250: PFLU_RS03250 - heavy-metal-associated domain-containing protein, at 751,368 to 751,565 _RS03250 PFLU_RS03255: PFLU_RS03255 - multidrug effflux MFS transporter, at 751,651 to 752,844 _RS03255 PFLU_RS03260: PFLU_RS03260 - hypothetical protein, at 752,853 to 753,035 _RS03260 PFLU_RS03265: PFLU_RS03265 - zinc-binding alcohol dehydrogenase family protein, at 753,182 to 754,195 _RS03265 PFLU_RS03270: PFLU_RS03270 - antibiotic biosynthesis monooxygenase, at 754,220 to 754,513 _RS03270
Group Condition PFLU_RS03250 PFLU_RS03255 PFLU_RS03260 PFLU_RS03265 PFLU_RS03270
pH Growth at pH8 and (C) Trisodium citrate -3.6 +0.1 -1.2 -0.6 -0.6
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -5.7 -0.1 +0.0 +0.1 -0.0
no stress control Glycine betaine (C)(N); with MOPS -5.8 -0.0 +0.3 +0.0 +0.4
carbon source acetate 30 mM (C) -3.3 +0.1 -0.7 -0.2 -0.6
carbon source propionate 20 mM (C) -1.4 +0.2 -0.0 -1.3 -0.9
carbon and nitrogen source NAG carbon and (N) -3.2 +0.1 +0.2 -0.1 -0.2
stress Betaine (C)(N); with MOPS; with chloride -2.2 +0.1 -0.6 -0.4 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.8 -0.2 -0.3 -0.5 +0.1
carbon source 4-Hydroxybenzoic Acid 2 mM (C) -2.4 -0.1 +0.2 -0.3 -0.1
pH Growth at pH7 and (C) D-Glucose -3.2 -0.1 +0.6 -0.1 +0.3
carbon source Shikimic Acid (C) -2.0 -0.3 +0.2 -0.0 -0.3
nitrogen source nitrate (N); with MOPS -2.2 +0.0 -0.3 +0.9 -0.6
carbon source Phloretic Acid 2 mM (C) -2.0 +0.3 -0.0 +0.2 -0.5
carbon source D,L-Malic Acid (C) -0.4 -0.1 -1.2 -0.6 +0.5
temperature Growth at 25C -1.8 +0.1 -0.1 +0.4 -0.4
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS -2.1 +0.1 -0.3 +0.3 +0.4
carbon source Inosine (C) -2.1 -0.0 -0.2 +0.5 +0.5
nitrogen source Ammonium chloride (N) -1.7 +0.1 +0.5 +0.3 -0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -2.3 +0.2 +0.3 -0.0 +0.8
stress Betaine (C)(N); with TAPS; with chloride -1.9 +0.2 +0.0 +0.1 +0.7
carbon source D-Glucuronic (C) -1.1 -0.2 +0.4 -0.6 +0.7
carbon source 4-Aminobutyric (C) -0.3 -0.1 +1.3 +0.3 -1.1
carbon source L-Valine (C) -1.6 -0.1 +0.8 +1.2 -0.0
stress Betaine (C)(N); with MOPS; with chloride +1.0 -0.1 +0.1 +0.6 -1.0
stress Betaine (C)(N); with MOPS; with chloride +2.0 -0.2 -0.7 +0.1 +0.0
carbon source L-Valine (C) +1.0 -0.2 +1.1 -0.3 -0.4
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +1.8 +0.2 +0.8 -0.5 -0.4
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS +1.1 +0.7 +0.6 -0.2 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +2.0 +0.1 +0.3 -0.1 +0.7
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.5 +0.2 +1.0 +0.7 +0.4
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