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Fitness for 5 genes in
Pseudomonas fluorescens SBW25-INTG
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500 nt
PFLU_RS02385 and PFLU_RS02390 overlap by 8 nucleotides
PFLU_RS02390 and PFLU_RS02395 are separated by 774 nucleotides
PFLU_RS02395 and PFLU_RS02400 are separated by 41 nucleotides
PFLU_RS02400 and PFLU_RS02405 overlap by 4 nucleotides
PFLU_RS02385: PFLU_RS02385 - bifunctional D-glycero-beta-D-manno-heptose-7-phosphate kinase/D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase HldE, at 547,362 to 548,786
_RS02385
PFLU_RS02390: PFLU_RS02390 - NAD-dependent epimerase/dehydratase family protein, at 548,779 to 549,705
_RS02390
PFLU_RS02395: PFLU_RS02395 - hypothetical protein, at 550,480 to 551,439
_RS02395
PFLU_RS02400: PFLU_RS02400 - aldo/keto reductase, at 551,481 to 552,293
_RS02400
PFLU_RS02405: PFLU_RS02405 - FAD-dependent oxidoreductase, at 552,290 to 553,465
_RS02405
Group
Condition
PFLU
_RS02385
PFLU
_RS02390
PFLU
_RS02395
PFLU
_RS02400
PFLU
_RS02405
carbon source
L-Ornithine (C)
N.D.
N.D.
-1.3
N.D.
N.D.
carbon source
L-Ornithine 10 mM (C)
N.D.
N.D.
-1.3
N.D.
N.D.
carbon source
L-Ornithine (C)
N.D.
N.D.
-1.1
N.D.
N.D.
carbon source
L-Ornithine (C)
N.D.
N.D.
-1.0
N.D.
N.D.
carbon source
L-Ornithine 10 mM (C)
N.D.
N.D.
-0.9
N.D.
N.D.
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
N.D.
N.D.
-0.9
N.D.
N.D.
carbon source
L-Ornithine 10 mM (C)
N.D.
N.D.
-0.8
N.D.
N.D.
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
N.D.
N.D.
-0.8
N.D.
N.D.
stress
Glucose (C) and L-Glutamine (N); with TAPS; with chloride
N.D.
N.D.
-0.7
N.D.
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
N.D.
N.D.
-0.6
N.D.
N.D.
stress
Glucose (C) and L-Glutamine (N); with TAPS; with chloride
N.D.
N.D.
-0.6
N.D.
N.D.
pH
Growth at pH7 and (C) D-Glucose
N.D.
N.D.
-0.6
N.D.
N.D.
stress
Betaine (C)(N); with MOPS; with chloride
N.D.
N.D.
-0.6
N.D.
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
N.D.
N.D.
-0.6
N.D.
N.D.
carbon source
NAG (C)
N.D.
N.D.
+0.6
N.D.
N.D.
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride
N.D.
N.D.
+0.6
N.D.
N.D.
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
N.D.
N.D.
+0.6
N.D.
N.D.
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
N.D.
N.D.
+0.6
N.D.
N.D.
carbon source
p-Coumaric (C) 5 mM
N.D.
N.D.
+0.7
N.D.
N.D.
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
N.D.
N.D.
+0.7
N.D.
N.D.
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
N.D.
N.D.
+0.7
N.D.
N.D.
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
N.D.
N.D.
+0.7
N.D.
N.D.
stress
malate (C) and Ammonium chloride (N); with MOPS; with PEG
N.D.
N.D.
+0.7
N.D.
N.D.
carbon source
p-Coumaric (C) 5 mM
N.D.
N.D.
+0.8
N.D.
N.D.
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
N.D.
N.D.
+0.8
N.D.
N.D.
carbon source
Citraconic 20 mM (C)
N.D.
N.D.
+0.8
N.D.
N.D.
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
N.D.
N.D.
+0.9
N.D.
N.D.
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
N.D.
N.D.
+0.9
N.D.
N.D.
carbon source
Citraconic 20 mM (C)
N.D.
N.D.
+1.0
N.D.
N.D.
carbon source
Citraconic 20 mM (C)
N.D.
N.D.
+1.0
N.D.
N.D.
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