Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS02320 and PFLU_RS02325 overlap by 1 nucleotidesPFLU_RS02325 and PFLU_RS02330 are separated by 61 nucleotidesPFLU_RS02330 and PFLU_RS02335 are separated by 6 nucleotidesPFLU_RS02335 and PFLU_RS02340 are separated by 75 nucleotides PFLU_RS02320: PFLU_RS02320 - sugar ABC transporter substrate-binding protein, at 530,588 to 531,568 _RS02320 PFLU_RS02325: PFLU_RS02325 - ATP-binding cassette domain-containing protein, at 531,568 to 531,816 _RS02325 PFLU_RS02330: PFLU_RS02330 - ABC transporter permease, at 531,878 to 532,804 _RS02330 PFLU_RS02335: PFLU_RS02335 - hypothetical protein, at 532,811 to 534,199 _RS02335 PFLU_RS02340: PFLU_RS02340 - TonB-dependent receptor, at 534,275 to 536,380 _RS02340
Group Condition PFLU_RS02320 PFLU_RS02325 PFLU_RS02330 PFLU_RS02335 PFLU_RS02340
pH Growth at pH9 and (C) D-Glucose -0.0 -0.8 +0.1 -0.3 -2.5
stress Growth with Chloride 200 mM +0.0 -0.8 +0.0 +0.3 -1.8
carbon source L-Isoleucine (C) -0.5 -1.2 -0.1 -0.1 +0.0
stress Growth with Chloride 200 mM +0.0 -0.1 -0.1 -0.2 -1.3
stress Growth with Chloride 200 mM -0.0 -0.3 +0.0 +0.0 -1.4
temperature shift Temperature shift 30_to_34 +0.0 -0.5 +0.0 +0.0 -1.3
stress Glucose (C) and L-Glutamine (N); with MOPS; with chloride -0.4 -0.9 -0.1 +0.0 -0.2
nitrogen source nitrite (N) -0.1 -0.7 +0.1 -0.0 -0.7
temperature Growth at 25C -0.1 -1.2 +0.2 +0.0 -0.3
carbon source Phloretic Acid 2 mM (C) -0.4 -1.7 +0.1 +0.5 +0.2
carbon source 4-Aminobutyric (C) +0.3 -1.0 -0.1 -0.1 -0.3
carbon source NAG (C) -0.3 -1.0 +0.2 -0.1 -0.1
temperature shift Temperature shift 30_to_34 +0.3 -0.0 -0.0 -0.1 -1.4
pH Growth at pH7 and (C) D-Glucose -0.7 -0.8 +0.1 +0.2 +0.2
no stress control Glucose (C) and nitrate (N); with TAPS +0.1 -1.2 +0.3 +0.0 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.4 -0.7 +0.1 -0.3 -0.4
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.2 -1.0 +0.0 +0.1 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.3 -1.0 -0.1 +0.0 +0.2
nitrogen source nitrate (N); with MOPS +0.3 -1.0 +0.1 +0.2 +0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.5 -0.3 -0.3 +0.2 +0.6
stress Betaine (C)(N); with MOPS; with chloride -0.1 -0.8 +0.2 +0.4 +0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.1 -0.9 -0.0 -0.0 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.1 -0.8 +0.3 +0.5 -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.2 -0.6 +0.2 -0.1 +0.6
pH Growth at pH8 and (C) Trisodium citrate -0.4 +0.7 -0.1 +0.2 +0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 -0.4 -0.1 -0.1 +0.8
carbon source L-Arabinose (C) +0.1 -0.8 +0.1 -0.1 +1.7
carbon source Shikimic Acid (C) +0.6 +0.6 +0.2 -0.2 -0.1
carbon source L-Arabinose (C) +0.1 -0.3 -0.1 -0.1 +1.8
carbon source L-Arabinose (C) +0.1 +0.1 -0.1 +0.1 +1.9
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