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Fitness for 5 genes in
Pseudomonas fluorescens SBW25-INTG
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PFLU_RS01035 and PFLU_RS01040 are separated by 155 nucleotides
PFLU_RS01040 and PFLU_RS01045 overlap by 30 nucleotides
PFLU_RS01045 and PFLU_RS01050 are separated by 108 nucleotides
PFLU_RS01050 and PFLU_RS01055 are separated by 224 nucleotides
PFLU_RS01035: PFLU_RS01035 - LysR family transcriptional regulator, at 231,095 to 232,039
_RS01035
PFLU_RS01040: PFLU_RS01040 - TonB-dependent receptor, at 232,195 to 234,510
_RS01040
PFLU_RS01045: PFLU_RS01045 - CynX/NimT family MFS transporter, at 234,481 to 235,668
_RS01045
PFLU_RS01050: PFLU_RS01050 - LysR family transcriptional regulator, at 235,777 to 236,643
_RS01050
PFLU_RS01055: PFLU_RS01055 - energy transducer TonB, at 236,868 to 237,683
_RS01055
Group
Condition
PFLU
_RS01035
PFLU
_RS01040
PFLU
_RS01045
PFLU
_RS01050
PFLU
_RS01055
no stress control
Glycine betaine (C)(N); with TAPS
+0.0
-0.1
-0.8
-0.3
-0.5
pH
Growth at pH8 and (C) Trisodium citrate
-0.1
-0.3
-1.0
-0.2
-0.1
temperature shift
Temperature shift 10_to_25; with MOPS
-0.4
-0.0
-0.8
-0.3
-0.0
carbon source
L-Alanine 15 mM (C)
+0.0
+0.0
-0.1
-0.3
-1.1
carbon source
m-Inositol (C)
+0.1
+0.1
-0.8
-0.5
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.1
+0.2
-0.7
-0.3
-0.5
carbon source
4-Guanidinobutyric (C)
-0.5
+0.0
+0.5
-0.5
-0.7
stress
malate (C) and Ammonium chloride (N); with TAPS
-0.4
+0.0
+0.2
-0.4
-0.3
no stress control
Glycine betaine (C)(N); with MOPS
+0.2
-0.0
-0.9
-0.2
-0.0
carbon source
p-Coumaric 2 mM (C)
-0.8
+0.0
-0.3
-0.2
+0.4
stress
Betaine (C)(N); with MOPS; with chloride
-0.5
+0.2
+0.1
-0.0
-0.6
carbon source
Inosine (C)
+0.5
+0.1
-0.5
-0.5
-0.3
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride
-0.3
+0.2
-0.5
-0.2
+0.5
temperature
Growth at 25C
-0.4
-0.0
+0.5
-0.4
-0.1
temperature
Growth at 25C
+0.5
+0.1
-0.6
-0.4
+0.1
carbon source
p-Coumaric 2 mM (C)
+0.2
-0.0
-0.7
+0.0
+0.4
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-0.3
-0.2
+0.5
+0.2
-0.4
carbon source
2-Deoxy-D-Ribose 10 mM (C)
-0.3
+0.1
+0.3
+0.6
-0.7
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
-0.3
+0.1
+0.3
+0.4
-0.4
stress
Betaine (C)(N); with MOPS; with chloride
+0.3
+0.0
-0.2
-0.4
+0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.3
-0.0
+0.5
-0.1
-0.5
stress
L-Glutamine (C)(N); with TAPS; with chloride
-0.1
+0.0
+0.6
+0.5
-0.8
carbon source
Phloretic Acid 2 mM (C)
+0.1
-0.1
+0.4
-0.4
+0.3
carbon source
4-hydroxyphenylacetic 2 mM (C)
+0.5
-0.0
-0.3
-0.2
+0.4
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-0.3
-0.0
-0.2
+0.3
+0.7
carbon source
L-Valine (C)
-0.2
-0.2
+0.3
+0.4
+0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.3
+0.0
+0.1
+0.6
-0.2
pH
Growth at pH7 and (C) D-Glucose
+0.3
-0.1
+0.5
+0.4
-0.2
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
+0.4
-0.1
-0.2
+0.2
+0.7
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
+0.3
+0.1
-0.3
+0.5
+0.8
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