Fitness for 5 genes in Enterobacter sp. TBS_079

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500 ntMPMX20_04300 and MPMX20_04301 are separated by 61 nucleotidesMPMX20_04301 and MPMX20_04302 are separated by 307 nucleotidesMPMX20_04302 and MPMX20_04303 are separated by 52 nucleotidesMPMX20_04303 and MPMX20_04304 are separated by 9 nucleotides MPMX20_04300: MPMX20_04300 - Putative ribose-phosphate pyrophosphokinase, at 4,485,285 to 4,485,968 _04300 MPMX20_04301: MPMX20_04301 - Fe/S biogenesis protein NfuA, at 4,486,030 to 4,486,605 _04301 MPMX20_04302: MPMX20_04302 - High-affinity gluconate transporter, at 4,486,913 to 4,488,229 _04302 MPMX20_04303: MPMX20_04303 - 4-alpha-glucanotransferase, at 4,488,282 to 4,490,363 _04303 MPMX20_04304: MPMX20_04304 - Maltodextrin phosphorylase, at 4,490,373 to 4,492,766 _04304
Group Condition MPMX20_04300 MPMX20_04301 MPMX20_04302 MPMX20_04303 MPMX20_04304
mixed community 0.1X LB plate, Mixed culture; Bacillus zanthoxyli TBS_109 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct -0.3 -2.3 +0.1 -1.6 -1.0
carbon source L-Sorbose (C) -1.7 -2.9 -0.2 +0.1 -0.2
mixed community AMD2036 coinoculated with AMD2590 on RCH2 to a starting OD of 0.001 -0.7 -3.9 +0.1 -0.1 -0.2
mixed community AMD2036 coinoculated with AMD2587 on LB to a starting OD of 0.001 -0.1 -3.8 -0.1 -0.3 -0.3
carbon source D-Maltose (C) -0.2 -0.7 -0.2 -1.5 -1.9
mixed community 0.1X LB plate, 1:1 coculture with Paenibacillus lautus TBS_090 -2.3 -1.2 +0.1 -0.3 -0.5
mixed community AMD2036 coinoculated with AMD2587 on LB to a starting OD of 0.01 -0.7 -3.1 +0.1 -0.4 -0.2
mixed community AMD2036 coinoculated with AMD2910 on LB to a starting OD of 0.001 -0.8 -3.4 +0.3 -0.1 +0.0
mixed community TSB plate, 1:1 coculture with Brevibacillus agri TBS_043 -0.7 -2.5 +0.2 -0.4 -0.4
mixed community Outgrowth from agar plate co-culture with Inquilinus sp. 005-A5 -0.3 -3.9 +0.1 +0.3 +0.1
nitrogen source Inosine (N) +0.4 -3.4 -0.1 -0.4 +0.0
mixed community 0.1X LB plate, Mixed culture; Streptomyces tendae SAI_182 1:1 starding OD to Enterobacter_TBS_079_ML3; Collection-direct -0.2 -3.1 -0.0 -0.1 +0.1
mixed community Outgrowth from agar plate co-culture with Achromobacter sp. TBS_048 -0.4 -3.2 +0.0 -0.1 +0.2
mixed community 0.1X LB plate, Mixed culture; Bacillus haynesii TBS_113 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct +0.3 -2.2 +0.1 -1.1 -0.4
supernatant mutant library grown in Supernatant; Enterobacter sp. TBS_079 grown in LB -0.0 -3.5 -0.0 -0.0 +0.2
mixed community 0.1X LB plate, Mixed culture; Bacillus zanthoxyli TBS_055 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct +0.7 -2.1 +0.5 -1.6 -0.8
mixed community TSB plate, 1:1 coculture with Flavobacterium anhuiense SO6b -0.2 -1.2 +0.2 -1.6 -0.5
mixed community AMD2036 coinoculated with AMD2590 on LB to a starting OD of 0.01 -0.1 -3.3 +0.0 +0.2 -0.0
mixed community 1X LB plate, 1:1 coculture with Paenibacillus lautus TBS_090 -0.3 -3.2 +0.4 -0.1 +0.1
mixed community control Growth on LB agar plate; mixed community control +0.5 -3.7 +0.1 +0.2 -0.1
mixed community 1X LB plate, 1:1 coculture with Promicromonospora thailandica SAI_039 +0.5 -2.9 -0.0 -0.5 +0.0
mixed community 0.1X LB plate, Mixed culture; Bacillus pumilus TBS_103 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct +0.3 -2.6 +0.2 -0.4 -0.4
supernatant mutant library grown in Supernatant; Inquilinus sp. 005-A5 grown in LB +0.5 -3.1 -0.1 +0.1 +0.2
mixed community 1X LB plate, 1:1 coculture with Bacillus megaterium TBS_037 +1.0 -2.7 +0.0 -0.1 -0.3
mixed community 0.1X LB plate, Mixed culture; Bacillus pumilus TBS_065 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct +2.4 -1.3 +0.1 -0.6 -0.3
mixed community M9 plate, 1:1 coculture with Pseudomonas silesiensis TBS_010 +0.3 -1.2 +0.4 +1.0 +0.9
in planta Plant=SorghumBicolor_Rtx430; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB_Kan50; Time=28_days; NumberPlantsPooled=4; CocultureWith=Inquilinus sp. 005-A5 +1.1 -1.9 -0.5 +0.3 +4.6
mixed community 0.1X LB plate, Mixed culture; Pseudomonas lini TBS_028 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct +0.1 +1.1 -0.2 +0.9 +3.2
in planta Plant=SorghumBicolor_Rtx430; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB_Kan50; Time=28_days; NumberPlantsPooled=4 -0.1 -2.2 +0.0 +2.2 +5.2
in planta Plant=SorghumBicolor_Rtx430; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB_Kan50; Time=28_days; NumberPlantsPooled=4 -0.2 -2.0 +0.2 +2.3 +5.4
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