Fitness for 5 genes in Methanococcus maripaludis S2

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500 ntMMP_RS04170 and MMP_RS04175 overlap by 8 nucleotidesMMP_RS04175 and MMP_RS08975 are separated by 258 nucleotidesMMP_RS08975 and MMP_RS04180 are separated by 196 nucleotidesMMP_RS04180 and MMP_RS04185 are separated by 11 nucleotides MMP_RS04170: MMP_RS04170 - DUF362 domain-containing protein, at 792,358 to 793,440 _RS04170 MMP_RS04175: MMP_RS04175 - permease, at 793,433 to 794,335 _RS04175 MMP_RS08975: MMP_RS08975 - hypothetical protein, at 794,594 to 794,749 _RS08975 MMP_RS04180: MMP_RS04180 - iron-containing alcohol dehydrogenase, at 794,946 to 796,115 _RS04180 MMP_RS04185: MMP_RS04185 - aldo/keto reductase, at 796,127 to 797,239 _RS04185
Group Condition MMP_RS04170 MMP_RS04175 MMP_RS08975 MMP_RS04180 MMP_RS04185
stress McCas_H2_complete Sulfaquinoxaline 0.1 mM -0.4 -0.2 -1.5 +0.1 +0.0
natural competence H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin -0.2 -0.3 -0.3 -0.2 -0.8
nutrient t2 H2 deepwell vitamin FREE -0.3 -0.0 -1.1 -0.1 -0.1
no stress control McCas_H2_complete no stress control -0.2 -0.2 -1.1 +0.1 -0.2
stress McCas_formate_complete 3-nitroxypropanol 0.000416666666666667 mM +0.1 -0.6 -0.8 +0.1 -0.1
nitrogen source H2 N-free with 5mM Inosine +0.2 -0.3 -0.9 -0.2 -0.1
stress McCas_H2_complete Hydroxylamine 1.04166666666667 mM -0.3 -0.1 -1.1 -0.2 +0.3
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations -0.1 +0.1 -0.4 -0.5 -0.5
stress McCas_formate_complete Rifampicin 0.5 mM -0.1 +0.2 -0.8 -0.2 -0.3
stress McCas_H2_complete Zinc Pyrithione 0.0142857142857143 mM -0.2 +0.2 -1.4 +0.1 +0.2
stress McCas_H2_complete Spiramycin 0.1 mM -0.0 +0.1 -0.9 -0.4 +0.1
stress McCas_H2_complete Bacitracin 0.003125 mM -0.1 +0.1 -1.2 -0.0 +0.2
stress McCas_H2_complete Cadmium chloride hemipentahydrate 0.555555555555556 mM +0.3 -0.2 -1.3 +0.0 +0.2
stress McCas_H2_complete Irgasan 0.025 mM -0.2 -0.0 -0.9 +0.3 -0.1
stress McCas_H2_complete Chlorate 250 mM +0.0 +0.2 -1.0 -0.3 +0.1
stress McCas_H2_complete Econazole nitrate salt 0.02 mM -0.3 +0.3 -0.9 +0.0 -0.0
no stress control McCas_H2_complete no stress control -0.3 +0.2 -1.0 +0.0 +0.3
stress McCas_formate_complete 3-nitroxypropanol 0.00833333333333333 mM -0.3 +0.3 -0.6 -0.3 +0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.5 -0.6 -0.5 -0.3 +0.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.3 -0.3 -0.7 -0.2 +0.2
nitrogen source H2 N-free with 10mM L-Methionine -0.4 -0.0 -0.7 -0.0 +0.5
stress McCas_H2_complete Hydroxylamine 1.04166666666667 mM -0.4 -0.3 +0.3 +0.2 +0.3
stress McCas_formate_complete Fluorophosphate 62.5 mM -0.4 +0.1 +0.6 +0.2 -0.1
nitrogen source Formate N-free with 2mM Adenosine -0.1 +0.3 +0.5 +0.1 -0.4
nitrogen source H2 N-free with 10mM Urea -0.0 +0.0 +1.1 -0.1 -0.3
stress McCas_formate_complete 1,2-dimethyl-5-nitroimidazole 0.00416666666666667 mM -0.3 +0.2 +0.9 -0.2 +0.2
stress McCas_formate_complete gold (III) chloride 0.0714285714285714 mM -0.2 +0.2 +0.9 -0.2 +0.0
nitrogen source H2 N-free with 10mM L-Serine -0.2 +0.3 +0.8 +0.1 -0.1
nitrogen source H2 N-free with 10mM L-tyrosine -0.1 +0.2 +1.5 -0.0 +0.1
stress McCas_formate_complete metavanadate 4.54545454545455 mM -0.1 +0.0 +2.5 +0.1 -0.2
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