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Fitness for 5 genes in
Methanococcus maripaludis S2
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500 nt
MMP_RS04170 and MMP_RS04175 overlap by 8 nucleotides
MMP_RS04175 and MMP_RS08975 are separated by 258 nucleotides
MMP_RS08975 and MMP_RS04180 are separated by 196 nucleotides
MMP_RS04180 and MMP_RS04185 are separated by 11 nucleotides
MMP_RS04170: MMP_RS04170 - DUF362 domain-containing protein, at 792,358 to 793,440
_RS04170
MMP_RS04175: MMP_RS04175 - permease, at 793,433 to 794,335
_RS04175
MMP_RS08975: MMP_RS08975 - hypothetical protein, at 794,594 to 794,749
_RS08975
MMP_RS04180: MMP_RS04180 - iron-containing alcohol dehydrogenase, at 794,946 to 796,115
_RS04180
MMP_RS04185: MMP_RS04185 - aldo/keto reductase, at 796,127 to 797,239
_RS04185
Group
Condition
MMP
_RS04170
MMP
_RS04175
MMP
_RS08975
MMP
_RS04180
MMP
_RS04185
stress
McCas_H2_complete Sulfaquinoxaline 0.1 mM
-0.4
-0.2
-1.5
+0.1
+0.0
natural competence
H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin
-0.2
-0.3
-0.3
-0.2
-0.8
nutrient t2
H2 deepwell vitamin FREE
-0.3
-0.0
-1.1
-0.1
-0.1
no stress control
McCas_H2_complete no stress control
-0.2
-0.2
-1.1
+0.1
-0.2
stress
McCas_formate_complete 3-nitroxypropanol 0.000416666666666667 mM
+0.1
-0.6
-0.8
+0.1
-0.1
nitrogen source
H2 N-free with 5mM Inosine
+0.2
-0.3
-0.9
-0.2
-0.1
stress
McCas_H2_complete Hydroxylamine 1.04166666666667 mM
-0.3
-0.1
-1.1
-0.2
+0.3
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
-0.1
+0.1
-0.4
-0.5
-0.5
stress
McCas_formate_complete Rifampicin 0.5 mM
-0.1
+0.2
-0.8
-0.2
-0.3
stress
McCas_H2_complete Zinc Pyrithione 0.0142857142857143 mM
-0.2
+0.2
-1.4
+0.1
+0.2
stress
McCas_H2_complete Spiramycin 0.1 mM
-0.0
+0.1
-0.9
-0.4
+0.1
stress
McCas_H2_complete Bacitracin 0.003125 mM
-0.1
+0.1
-1.2
-0.0
+0.2
stress
McCas_H2_complete Cadmium chloride hemipentahydrate 0.555555555555556 mM
+0.3
-0.2
-1.3
+0.0
+0.2
stress
McCas_H2_complete Irgasan 0.025 mM
-0.2
-0.0
-0.9
+0.3
-0.1
stress
McCas_H2_complete Chlorate 250 mM
+0.0
+0.2
-1.0
-0.3
+0.1
stress
McCas_H2_complete Econazole nitrate salt 0.02 mM
-0.3
+0.3
-0.9
+0.0
-0.0
no stress control
McCas_H2_complete no stress control
-0.3
+0.2
-1.0
+0.0
+0.3
stress
McCas_formate_complete 3-nitroxypropanol 0.00833333333333333 mM
-0.3
+0.3
-0.6
-0.3
+0.2
nutrient t2
H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
+0.5
-0.6
-0.5
-0.3
+0.1
nutrient t2
H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
+0.3
-0.3
-0.7
-0.2
+0.2
nitrogen source
H2 N-free with 10mM L-Methionine
-0.4
-0.0
-0.7
-0.0
+0.5
stress
McCas_H2_complete Hydroxylamine 1.04166666666667 mM
-0.4
-0.3
+0.3
+0.2
+0.3
stress
McCas_formate_complete Fluorophosphate 62.5 mM
-0.4
+0.1
+0.6
+0.2
-0.1
nitrogen source
Formate N-free with 2mM Adenosine
-0.1
+0.3
+0.5
+0.1
-0.4
nitrogen source
H2 N-free with 10mM Urea
-0.0
+0.0
+1.1
-0.1
-0.3
stress
McCas_formate_complete 1,2-dimethyl-5-nitroimidazole 0.00416666666666667 mM
-0.3
+0.2
+0.9
-0.2
+0.2
stress
McCas_formate_complete gold (III) chloride 0.0714285714285714 mM
-0.2
+0.2
+0.9
-0.2
+0.0
nitrogen source
H2 N-free with 10mM L-Serine
-0.2
+0.3
+0.8
+0.1
-0.1
nitrogen source
H2 N-free with 10mM L-tyrosine
-0.1
+0.2
+1.5
-0.0
+0.1
stress
McCas_formate_complete metavanadate 4.54545454545455 mM
-0.1
+0.0
+2.5
+0.1
-0.2
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